Comparative gene expression profiling of human PGP1 iPS, lymphocyte, donor fibroblasts
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Je Lee
PROVIDER: E-GEOD-54733 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Highly multiplexed subcellular RNA sequencing in situ.
Lee Je Hyuk JH Daugharthy Evan R ER Scheiman Jonathan J Kalhor Reza R Yang Joyce L JL Ferrante Thomas C TC Terry Richard R Jeanty Sauveur S F SS Li Chao C Amamoto Ryoji R Peters Derek T DT Turczyk Brian M BM Marblestone Adam H AH Inverso Samuel A SA Bernard Amy A Mali Prashant P Rios Xavier X Aach John J Church George M GM
Science (New York, N.Y.) 20140227 6177
Understanding the spatial organization of gene expression with single-nucleotide resolution requires localizing the sequences of expressed RNA transcripts within a cell in situ. Here, we describe fluorescent in situ RNA sequencing (FISSEQ), in which stably cross-linked complementary DNA (cDNA) amplicons are sequenced within a biological sample. Using 30-base reads from 8102 genes in situ, we examined RNA expression and localization in human primary fibroblasts with a simulated wound-healing assa ...[more]
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