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Genome-wide replication analysis of a yeast eco1 mutant by deep sequencing.

ABSTRACT: Eco1 is the acetyltransferase that establishes sister-chromatid cohesion during DNA replication. Budding yeast with an eco1 mutation that genocopies Roberts syndrome displaysreduced ribosomal DNA (rDNA) transcription and a transcriptional signature of starvation. Weshow that deleting FOB1, a gene encoding a specific replication fork blocking protein for therDNA region, rescues rRNA production and partially rescues transcription genome-wide. This experiment examines the effect of eco1 mutation on replication genome-wide. Furtherstudies show that deletion of FOB1 corrects the genome-wide replication defects, nucleolarstructure, and rDNA segregation in an eco1 mutant. Our study highlights cohesin's central role atthe rDNA for global control of DNA replication and gene expression. DNA content in eco1-W216G mutant and wt yeast is measured in duplicate by sequencing at 0, 20, and 40 minutes following release from G1 arrest.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Chris Seidel 

PROVIDER: E-GEOD-54742 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The cohesin acetyltransferase Eco1 coordinates rDNA replication and transcription.

Lu Shuai S   Lee Kenneth K KK   Harris Bethany B   Xiong Bo B   Bose Tania T   Saraf Anita A   Hattem Gaye G   Florens Laurence L   Seidel Chris C   Gerton Jennifer L JL  

EMBO reports 20140314 5

Eco1 is the acetyltransferase that establishes sister-chromatid cohesion during DNA replication. A budding yeast strain with an eco1 mutation that genocopies Roberts syndrome has reduced ribosomal DNA (rDNA) transcription and a transcriptional signature of starvation. We show that deleting FOB1--a gene that encodes a replication fork-blocking protein specific for the rDNA region--rescues rRNA production and partially rescues transcription genome-wide. Further studies show that deletion of FOB1 c  ...[more]

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