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Gene epxression profiling in murine Flt3+ granulocyte progenitors (Flt3+ GPs) and Flt3- granulocyte progenitors (Flt3- GPs)

ABSTRACT: To understand the mechanism by which IRF8 promotes basophil differentiation Murine bone marrow Flt3+ GPs and Flt3- GPs were isolated by cell sorting from wild-type or Irf8 deficient mice. Two independent experiments were performed.

ORGANISM(S): Mus musculus

SUBMITTER: Tomohiko Tamura

PROVIDER: E-GEOD-54887 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene epxression profiling in murine Flt3+ granulocyte progenitors (Flt3+ GPs) and Flt3- granulocyte progenitors (Flt3- GPs)

Project description:To understand the mechanism by which IRF8 promotes basophil differentiation

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Project description:To understand the mechanism by which IRF8 inhibits neutrophil differentiation Murine bone marrow MDPs and cMoPs were isolated by cell sorting from wild-type or Irf8 deficient mice. 32Dcl.3 cells were transduced with retroviruses carrying murine C/EBPalpha cDNA and/or murine Irf8 cDNA. Two independent experiments were performed.

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Project description:To understand the effect of BCR-ABL and IRF8 on gene expression pattern during in vitro dendritic cell differentiation, microarry analysis was performed. Murine bone marrow Lin- cells were transduced with retroviruses carrying human BCR-ABL cDNA and/or mouse Irf8 cDNA, and then cultured with Flt3L for 5 days. Two independent experiments were performed.

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The Granulocyte Progenitor Stage is a Key Target of IRF8-Mediated Regulation of Myeloid-Derived Suppressor Cell Production

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The Granulocyte Progenitor Stage is a Key Target of IRF8-Mediated Regulation of Myeloid-Derived Suppressor Cell Production

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A combined gas phase separation strategy for ADP-ribosylated peptides

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