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Map Brachyury-bound enhancers by ChIP-seq

ABSTRACT: To gain new mechanistic insights into highly-regulated lineage specification and morphogenetic processes during early embryogenesis, we applied ChIP-seq to identify transcriptional programs mediated by a key developmental regulator - Brachyury. Embryonic stem cells were differentiated into Brachyury-positive mesoendoderm cells. And, ChIP-seq experiments were carried out by two independent Brachyury antibodies.

ORGANISM(S): Mus musculus

SUBMITTER: Zhe Liu

PROVIDER: E-GEOD-54978 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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High-resolution Brachyury DNA-binding Sites Mapping by ChIP-exo

Project description:Mapping ultra high resolution of Brachyury:DNA interaction would provide us with valuable new mechanistic insights into complex molecular transactions at Brachyury-bound enhancers. Embryonic stem cells were differentiated into Brachyury-positive mesoendoderm cells. And, ChIP-exo experiment was then performed to identify detailed Brachyury-DNA binding profiles.

2014-03-10 | E-GEOD-54963 | biostudies-arrayexpress

Map Brachyury-bound enhancers by ChIP-seq

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Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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Targets of the transcription factor Brachyury in differentiating mouse ES cells

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