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Regulation of S-phase transcription factors by Cdk1

ABSTRACT: Analysis of gene expression across the cell cycle from wild type cells, and cells expressing alleles of Yox1, Yhp1, Hcm1, and Tos4 that cannot be phosphorylated by Cdk1. Expression of S-phase and M/G1 transcripts are downregulated when phosphorylation of these factors is blocked, demonstrating that Cdk1 promotes expression of late cell cycle genes. These experiments are two-color hybridizations of RNA isolated from synchronized wild type (WT) or phosphomutant (4P) cells, compared to RNA from asyncrhonous wild type cells in mid-log phase. Wild type and mutant cells were synchronized in G1 phase, released into the cell cycle and samples collected at 15 minute intervals. Each time course was carried out in duplicate, the replicate experiment was performed as a dye swap.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Jennifer Benanti

PROVIDER: E-GEOD-55121 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Regulation of a transcription factor network by Cdk1 coordinates late cell cycle gene expression.

Landry Benjamin D BD Mapa Claudine E CE Arsenault Heather E HE Poti Kristin E KE Benanti Jennifer A JA

The EMBO journal 20140408 9

To maintain genome stability, regulators of chromosome segregation must be expressed in coordination with mitotic events. Expression of these late cell cycle genes is regulated by cyclin-dependent kinase (Cdk1), which phosphorylates a network of conserved transcription factors (TFs). However, the effects of Cdk1 phosphorylation on many key TFs are not known. We find that elimination of Cdk1-mediated phosphorylation of four S-phase TFs decreases expression of many late cell cycle genes, delays mi ...[more]

PMID: 24714560

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