Ontology highlight
ABSTRACT:
ORGANISM(S): Mus musculus
SUBMITTER: Yue Zhang
PROVIDER: E-GEOD-55131 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Nucleic acids research 20140930 19
The use of RNA interference is becoming routine in scientific discovery and treatment of human disease. However, its applications are hampered by unwanted effects, particularly off-targeting through miRNA-like pathways. Recent studies suggest that the efficacy of such off-targeting might be dependent on binding stability. Here, by testing shRNAs and siRNAs of various GC content in different guide strand segments with reporter assays, we establish that weak base pairing in both seed and 3' region ...[more]