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RNA-Seq of Aged Polg Mutator Mouse Brains

ABSTRACT: We have performed both proteomic and transcriptomic analyses of the brains of one-year old Polg mutator mice. As part of this analysis, we generated RNA-Seq data from RNA isolated from one cerebral hemisphere of these mice. mRNA levels and protein abundance levels were compared in this study. 11 samples of RNA isolated from mouse cerebral hemisphere. 7 Polg D257A/D257A, 1 Polg D257A/WT, and 3 Polg WT/WT. The samples were collected from one-year old male mice.

ORGANISM(S): Mus musculus

SUBMITTER: Jinhui Ding

PROVIDER: E-GEOD-55188 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Post-translational decrease in respiratory chain proteins in the Polg mutator mouse brain.

Hauser David N DN Dillman Allissa A AA Ding Jinhui J Li Yan Y Cookson Mark R MR

PloS one 20140410 4

Mitochondrial DNA damage is thought to be a causal contributor to aging as mice with inactivating mutations in polymerase gamma (Polg) develop a progeroid phenotype. To further understand the molecular mechanisms underlying this phenotype, we used iTRAQ and RNA-Seq to determine differences in protein and mRNA abundance respectively in the brains of one year old Polg mutator mice compared to control animals. We found that mitochondrial respiratory chain proteins are specifically decreased in abun ...[more]

PMID: 24722488

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