Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression analysis of Cbfb-deficient LSK and GMP


ABSTRACT: Runx/Cbfb heterodimers play important roles in the development of hematopoietic cells in mouse embryos and adults. In order to identify genes that are regulated by Runx/Cbfb, we purified Lin– c-kit+ Sca1+ (LSK) cells and Lin– c-kit+ Sca1– CD16/32+ (GMP) cells from Vav1-iCre x Cbfb(F/F) and Vav1-iCre x Cbfb(F/+) mice and profiled gene expression using microarray. 200,000 LSK and GMP cells were purified separately from two 7 week old Vav1-iCre x Cbfb(F/F) mice and two Vav1-iCre x Cbfb(F/+) mice by cell sorting. The purity was higher than 98%. Total RNA was extracted using the NucleoSpin RNA XS kit (Macherey-Nagel), amplified using a PicoSL RNA amplification kit (Nugen) and biotinylated with Encore biotin module (Nugen). Labeled RNA was hybridized to Mouse Gene 1.0ST microarrays (Affymetrix) according to the manufacturer’s instruction.

ORGANISM(S): Mus musculus

SUBMITTER: Takeshi Egawa 

PROVIDER: E-GEOD-55227 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Runx1 and Cbfβ regulate the development of Flt3+ dendritic cell progenitors and restrict myeloproliferative disorder.

Satpathy Ansuman T AT   Briseño Carlos G CG   Cai Xiongwei X   Michael Drew G DG   Chou Chun C   Hsiung Sunnie S   Bhattacharya Deepta D   Speck Nancy A NA   Egawa Takeshi T  

Blood 20140327 19


Runx1 and Cbfβ are critical for the establishment of definitive hematopoiesis and are implicated in leukemic transformation. Despite the absolute requirements for these factors in the development of hematopoietic stem cells and lymphocytes, their roles in the development of bone marrow progenitor subsets have not been defined. Here, we demonstrate that Cbfβ is essential for the development of Flt3(+) macrophage-dendritic cell (DC) progenitors in the bone marrow and all DC subsets in the peripher  ...[more]

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