Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Genome wide occupancy of Msgn1 in differentiating embryonic stem cells


ABSTRACT: Msgn1 is a bHLH transcription factor and is a direct target gene of the Wnt/b-catenin signaling pathway. During mouse embryogenesis, Msgn1 is expressed in the mesodermal compartment of the primitive streak and is required for the differentiation of presomitic mesoderm. Msgn1-/- mutants show defects in somitogenesis leading to a lack of trunk skeletal muscles, vertebra and ribs. The goal of this study is to dissect the molecular and cellular function of Msgn1 in Embryonic Stem Cells (ESC) and mouse development. In order to identify direct Msgn1 targets, we performed transcriptional profiling and CHIP-seq of Msgn1 expressing differentiating ES cells. Integration of these data sets, we found that Msgn1 is a master regulator of PSM differentiation regulating gene expression programs of PSM identity, EMT, motility and Notch segmentation clock. Inducible Flag Msgn1 ES cells were differentiated to form Embryoid bodies (EBs) for 2 days. Flag-Msgn1 was induced on day 2 with doxycycline and samples were collected 36h later. Here Input DNA is used as control.

ORGANISM(S): Mus musculus

SUBMITTER: Ravindra B Chalamalasetty 

PROVIDER: E-GEOD-55263 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

Mesogenin 1 is a master regulator of paraxial presomitic mesoderm differentiation.

Chalamalasetty Ravindra B RB   Garriock Robert J RJ   Dunty William C WC   Kennedy Mark W MW   Jailwala Parthav P   Si Han H   Yamaguchi Terry P TP  

Development (Cambridge, England) 20141101 22


Neuromesodermal (NM) stem cells generate neural and paraxial presomitic mesoderm (PSM) cells, which are the respective progenitors of the spinal cord and musculoskeleton of the trunk and tail. The Wnt-regulated basic helix-loop-helix (bHLH) transcription factor mesogenin 1 (Msgn1) has been implicated as a cooperative regulator working in concert with T-box genes to control PSM formation in zebrafish, although the mechanism is unknown. We show here that, in mice, Msgn1 alone controls PSM differen  ...[more]

Similar Datasets

2011-07-01 | E-GEOD-29848 | biostudies-arrayexpress
2014-09-04 | GSE55263 | GEO
2015-01-01 | GSE39614 | GEO
2018-03-05 | GSE111391 | GEO
2015-08-03 | GSE71348 | GEO
2015-08-03 | E-GEOD-71348 | biostudies-arrayexpress
2015-01-01 | E-GEOD-39613 | biostudies-arrayexpress
2015-01-01 | E-GEOD-39614 | biostudies-arrayexpress
2021-07-21 | E-MTAB-9773 | biostudies-arrayexpress
2014-04-16 | E-GEOD-37151 | biostudies-arrayexpress