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Influence of Cochliobolus miyabeanus and silicon on gene expression in rice leaves (Oryza sativa L.)

ABSTRACT: Although Cochliobolus miyabeanus is an important fungal leaf pathogen on rice plants worldwide, it is largely neglected by molecular plant phytopathologists. To shed new light on the molecular and genetic basis of the rice M-bM-^@M-^S C. miyabeanus interaction, we compared the transcriptome of rice leaves 12h post inoculation to uninfected leaves. Even though usable sources of resistance against brown spot disease caused by C. miyabeanus are scarce, silicon application emerges as a sustainable protection strategy. Many articles report the beneficial effect of silicon on brown spot resistance. however the underlying mechanisms remain largely unclear. The influence of silicon application on the transcriptome of healthy and infected rice leaves 12hpi was compared as well in an attempt to disentangle the modulation of silicon-induced brown spot resistance. Comparison between C. miyabeanus- and mock-infected rice leaves 12h post inoculation. This study consist of a 2 x 2 factorial design (infected and non-infected; untreated and silicon-treated) in three biological replicates.

ORGANISM(S): Oryza sativa

SUBMITTER: Jonas Van Bockhaven

PROVIDER: E-GEOD-55330 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Influence of Cochliobolus miyabeanus and silicon on gene expression in rice leaves (Oryza sativa L.)

Project description:Although Cochliobolus miyabeanus is an important fungal leaf pathogen on rice plants worldwide, it is largely neglected by molecular plant phytopathologists. To shed new light on the molecular and genetic basis of the rice – C. miyabeanus interaction, we compared the transcriptome of rice leaves 12h post inoculation to uninfected leaves. Even though usable sources of resistance against brown spot disease caused by C. miyabeanus are scarce, silicon application emerges as a sustainable protection strategy. Many articles report the beneficial effect of silicon on brown spot resistance. however the underlying mechanisms remain largely unclear. The influence of silicon application on the transcriptome of healthy and infected rice leaves 12hpi was compared as well in an attempt to disentangle the modulation of silicon-induced brown spot resistance.

2014-02-25 | GSE55330 | GEO

Transcription profiling of rice plants infected by rice blast fungus

Project description:To investigate plant-fungus interactions in early stage of infection, we analyzed response of rice against Magnaporthe grisea infection deficient mutants. In M. grisea, Mgb1 and Mst12 are essential for development of infection structures. Deletion of MGB1 results in defect in appresorium formation, and MST12, in penetration peg development. Analysis of gene expression profiles in rice by microarray revealed the mutant-specific and R gene dependent gene expression, strongly suggesting that gene-for-gene interaction commences before the penetration into rice cell. Experiment Overall Design: Agilent rice oligo microarray was used to investigate the gene expression profiling in rice plants infected with Guy11, Mgb1 and Mst12. Total RNAs were isolated from fourth leaves at 24 and 48 hpi. Agilent rice DNA chips were used according to the manufactureâs protocol. After hybridization, arrays were scanned using an Agilent Microarray Scanner, and Feature Extraction version 9.1 was used for image analysis and data extraction. Signal intensities derived from inoculated leaved were compared with that of mock-inoculated leaves. In each treatment, the experiment was performed independently three times.

2008-06-16 | E-GEOD-9450 | biostudies-arrayexpress

Gene expression in rice leaves (Pia) in response to inoculation with rice blast fungus

Project description:Fourth leaves of rice seedlings (4.5 leaf stage) grown in hydroponic culture were inoculated with rice blast fungus and gene expression profiles were analyzed by microarray. Fourth leaves of the two isogenic lines of rice cv Nipponbare (blast-resistance gene: Pia or its mutant, pia) were inoculated with rice blast fungus, P91-15B, carrying avirulence gene, AvrPia. Total RNA was isolated, labeled with cy3, and probed with agilent rice oligoarray (4x44).

2015-04-01 | E-GEOD-62893 | biostudies-arrayexpress

Gene expression in rice leaves (Pish) in response to inoculation with rice blast fungus

Project description:Fourth leaves of rice seedlings (4.5 leaf stage) grown in hydroponic culture were inoculated with rice blast fungus and gene expression profiles were analyzed by microarray. Fourth leaves of the two isogenic lines of rice cv Nipponbare (blast-resistance gene: Pish or its mutant, pish) were inoculated with rice blast fungus, Kyu77-07A, carrying avirulence gene, AvrPish. Total RNA was isolated, labeled with cy3, and probed with agilent rice oligoarray (4x44).

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Gene expression in invaded cells and the flanking cells of rice in response to the inoculation with rice blast fungus

Project description:Rice blast fungus was inoculated to susceptible and resistant lines of rice leaves and the invaded cells and their adjacent cells were collected by Laser Microdissection method. The extracted RNA was amplified and analyzed by two color microarray. Two-condition experiment, incompatible (resistant) and compatible (susceptible) combination by using two isogenic lines of rice cv Nipponbare. Biological replicates: 3 replicates. Time course: 4 points.

2015-02-19 | E-GEOD-62422 | biostudies-arrayexpress

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Rice gene global expression during nonhost interaction with Blumeria graminis f. sp. hordei (Bgh)

Project description:Powdery mildew is a very common plant disease and only few plants are immune. Host interactions have been identified and characterized for the pathosystems barley-B. graminis f. sp. tritici (Bgt) and wheat-B. graminis f. sp. hordei (Bgh), whereas no data are reported about powdery mildew and nonhost plants, such as rice. On the other hand rice nonhost resistance is widely unexploited and only few expression data are available. To characterize rice response during nonhost interaction with Bgh, a global expression analysis was performed by using the GeneChipM-BM-. Rice Genome Array. To describe rice gene expression profiles during nonhost interaction, 2 week-old rice plantlets were inoculated with Bgh. Treated (inoculated) and control (mock) samples were collected 24 hours post-inoculation for GeneChipM-BM-. Rice Genome Array hybridization. For transcript proM-oM-,M-^Aling experiments with powdery mildew, entire leaves were sampled from rice plantlets 2 weeks old (cv. Nipponbare). Treated and control (mock) rice leaves were collected 24 hours post inoculation. Three biological replicates for inoculated and control plants were extracted and analysed independently with the GeneChipM-BM-. Rice Genome Array.

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WRKY type transcription factor OsWRKY28 overexpression effect on rice leaves 48 h after Magnaporthe Oryzae infection

Project description:Analysis of transgenic rice overexpressing OsWRKY28, a WRKY type transcription factor. Results provide insight into the role of OsWRKY28 in the defense signaling against rice blast fungus. Expression profiling in wild-type and OsWRKY28 overexpressing rice leaves infected with or without Magnaporthe Oryzae was analyzed using one-color method with three biological replicates.

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