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Transcription profiling of Arabidopsis leaves to identify targets of AtWRKY27 in

ABSTRACT: The Arabidopsis transcription factor WRKY27 was found to be involved in plant defense towards Ralstonia solanacearum GMI1000. To identify target genes of WRKY27, we introduced a functional tagged version of WRKY27 into two independent Arabidopsis wrky27 knockout lines (ecotype Columbia) under the control of the estrogen receptor-based chemical-inducible system. 18 days old plants grown on soil (Metro Mix 200) at 22oC under a 10/14 h light/dark cycle were treated for 6h with 10 microM beta-estradiol after which RNA was immediately isolated. The two independent knockout lines transformed with the empty vectors (pMD::XVE-SALK and pMD::XVE-ETL) served as controls and were grown under the same conditions and treated identically as the experimental plants. Experimenter name = Shahid Mukhtar; Experimenter phone = +49-221-5062-310; Experimenter fax = +49-221-5062-353; Experimenter address = Max.Planck-Institute for Plant Breeding; Experimenter address = Dept. Plant Microbe Interactions; Experimenter address = Carl-von-Linne Weg 10; Experimenter address = Koeln; Experimenter zip/postal_code = 50829; Experimenter country = Germany Experiment Overall Design: 4 samples were used in this experiment

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Nottingham Arabidopsis Stock Centre (NASC)

PROVIDER: E-GEOD-5537 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Targets of AtWRKY27 in Arabidopsis leaves

Project description:The Arabidopsis transcription factor WRKY27 was found to be involved in plant defense towards Ralstonia solanacearum GMI1000. To identify target genes of WRKY27, we introduced a functional tagged version of WRKY27 into two independent Arabidopsis wrky27 knockout lines (ecotype Columbia) under the control of the estrogen receptor-based chemical-inducible system. 18 days old plants grown on soil (Metro Mix 200) at 22oC under a 10/14 h light/dark cycle were treated for 6h with 10 microM beta-estradiol after which RNA was immediately isolated. The two independent knockout lines transformed with the empty vectors (pMD::XVE-SALK and pMD::XVE-ETL) served as controls and were grown under the same conditions and treated identically as the experimental plants. Experimenter name = Shahid Mukhtar Experimenter phone = +49-221-5062-310 Experimenter fax = +49-221-5062-353 Experimenter address = Max.Planck-Institute for Plant Breeding Experimenter address = Dept. Plant Microbe Interactions Experimenter address = Carl-von-Linne Weg 10 Experimenter address = Koeln Experimenter zip/postal_code = 50829 Experimenter country = Germany Keywords: genetic_modification_design

2006-08-15 | GSE5537 | GEO

Group II-A WRKY transcription factors and early leaf senescence (2)

Project description:In our laboratory we are interested in studying the functions of WRKY zink finger type transcription factors. There are 74 members of this gene family in Arabidopsis. WRKY factors are key regulators of distinct plant defense responses and are involved in certain developmental programs e.g. plant senescence. We would like to determine the functions of a small sub-group (group II-a) of WRKY factors. Our aim to compare and contrast the gene expression profiles of 35 days-old untreated wild type and WRKY T-DNA knockout plants grown in a growth chamber under long day growth conditions. All plants chosen at this stage showed slight yellowing of the first two to four leaves. Experimenter name: Bekir Uelker Experimenter phone: 49-221-5062-310 Experimenter fax: 49-221-5062-353 Experimenter department: Somsissich Lab Experimenter institute: Max-Planck-Institute for Plant Breeding Research Experimenter address: Department of Plant Microbe Interactions Experimenter address: Carl-von-Linne-Weg 10 Experimenter address: Cologne Experimenter zip/postal_code: 50829 Experimenter country: Germany Keywords: genetic_modification_design

2007-01-22 | GSE6830 | GEO

Group II-A WRKY transcription factors and early leaf senescence

Project description:In our laboratory we are interested in studying the functions of WRKY zinc finger type transcription factors. There are 74 members of this gene family in Arabidopsis. WRKY factors are key regulators of distinct plant defense responses and are involved in certain developmental programs e.g. trichome development and plant leaf senescence. We would like to determine the functions of a small sub-group (group II-a) of WRKY factors in response to bacterial infection. Our aim is to compare and contrast the gene expression profiles of 3-week-old wild type and WRKY T-DNA knockout mutants grown in a growth chamber under long day growth conditions and subsequently challenged for 6 hours with the virulent bacterial pathogen /Pseudomonas syringae/ DC3000. Experimenter name: Bekir Uelker Experimenter phone: 49-221-5062-310 Experimenter fax: 49-221-5062-353 Experimenter department: Somsissich Lab Experimenter institute: Max-Planck-Institute for Plant Breeding Research Experimenter address: Department of Plant Microbe Interactions Experimenter address: Carl-von-Linne-Weg 10 Experimenter address: Cologne Experimenter zip/postal_code: 50829 Experimenter country: Germany Keywords: genetic_modification_design

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Transcription profiling of Arabidopsis Group II-A WRKY transcription factor knockouts - early leaf senescence (2)

Project description:In our laboratory we are interested in studying the functions of WRKY zink finger type transcription factors. There are 74 members of this gene family in Arabidopsis. WRKY factors are key regulators of distinct plant defense responses and are involved in certain developmental programs e.g. plant senescence. We would like to determine the functions of a small sub-group (group II-a) of WRKY factors. Our aim to compare and contrast the gene expression profiles of 35 days-old untreated wild type and WRKY T-DNA knockout plants grown in a growth chamber under long day growth conditions. All plants chosen at this stage showed slight yellowing of the first two to four leaves. Experimenter name: Bekir Uelker; Experimenter phone: 49-221-5062-310; Experimenter fax: 49-221-5062-353; Experimenter department: Somsissich Lab; Experimenter institute: Max-Planck-Institute for Plant Breeding Research; Experimenter address: Department of Plant Microbe Interactions; Experimenter address: Carl-von-Linne-Weg 10; Experimenter address: Cologne; Experimenter zip/postal_code: 50829; Experimenter country: Germany Experiment Overall Design: 8 samples were used in this experiment

2008-06-15 | E-GEOD-6830 | biostudies-arrayexpress

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2008-06-15 | E-GEOD-6829 | biostudies-arrayexpress

FRI FLC combos

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2007-01-08 | GSE5746 | GEO

To identify changes in gene expression during silique senescence in Arabidopsis thaliana

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2007-01-08 | GSE5736 | GEO

Transcription profiling of Arabidopsis flc3/fri mutants under different light conditions

Project description:This experiment was annotated by TAIR (http://arabidopsis.org).FRI FLC time series; Experimenter name = Markus Schmid; Experimenter phone = ++49-7071-601-1413; Experimenter fax = ++49-7071-601-1412; Experimenter department = Detlef Weigel Laboratory Germany; Experimenter institute = Max-Planck-Institute for Developmental Biology; Experimenter address = Dept. of Molecular Biology; Experimenter address = Spemannstr. 37-39; Experimenter address = TÃ¼bingen; Experimenter zip/postal_code = 72076; Experimenter country = Germnay Experiment Overall Design: 36 samples were used in this experiment

2008-06-14 | E-GEOD-5746 | biostudies-arrayexpress

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2007-01-22 | GSE6825 | GEO

Transcription profiling of Arabidopsis to identify gene expression during silique senescence

Project description:The aim of the experiment is to compare and contrast the profiles of gene expression during silique senescence and to identify the molecular events that regulate the timing of the process. Silique occurs over a highly predictable time frame and as a consequence it may be easier to identify the molecular events that are critical for the process to take place than in systems such as leaf senescence. The material will be collected from 20 Arabidopsis thaliana ecotype Columbia plants (NASC order number N1093) grown under controlled conditions specified under NASC protocol: Baseline sample preparation. Two pods will be collected from each plant and pooled from 20 plants to reduce sampling variability. It is intended that 3 replicates of the experiment will be submitted. The RNA will be extracted from mature green silique tissues of 10 days after anthesis and senescing pod tissues of 20 days after anthesis using lithium chloride extraction protocol and cleaned with Qiagen RNAeasy column. Experimenter name = Thomas Yang; Experimenter phone = 01159516378; Experimenter institute = University of Nottingham; Experimenter address = Division of Plant Sciences; Experimenter address = University of Nottingham; Experimenter address = Sutton Bonington Campus; Experimenter address = Loughborough; Experimenter address = Leicestershire; Experimenter zip/postal_code = LE12 5RD; Experimenter country = UK Experiment Overall Design: 6 samples were used in this experiment

2008-06-14 | E-GEOD-5736 | biostudies-arrayexpress

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