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Whole transcription data for mouse spermatogonial cells

ABSTRACT: Spermatogenesis is a multi-step yet tightly regulated developmental process to produce male gemetes for reproduction. In mouse spermatogenesis, a sub-group of type A spermatogonia (Spga) known as spermatogonial stem cells (SSCs) maintain their population by self-renewal, and differentiate through mitosis and meiosis to form tetraploid (4n) pacchytene spermatocytes (Spcy) and haploid (n) round spermatids (Sptd), which further differentiate into functional sperms. In this study, we selected three representative stages of mouse spermatogenesis, namely Spga, Spcy and Sptd, to study their transcriptomic charteristics using whole genome tiling microarray. Three biological replicates of each selected stage. Spga were isolated from 6-day-old Balb/c mice, Spcy and Sptd from 60-day-old Balb/c mice. Transcriptomic landscapes of these three cell types were analysed with whole genome tiling array.

ORGANISM(S): Mus musculus

SUBMITTER: Tin-Lap Lee

PROVIDER: E-GEOD-55381 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Revealing the transcriptome landscape of mouse spermatogonial cells by tiling microarray.

Lee Tin-Lap TL Rennert Owen M OM Chan Wai-Yee WY

Methods in molecular biology (Clifton, N.J.) 20120101

In the past decade, the advent of microarray technologies has allowed functional genomic studies of male germ cell development, resulting in the identification of genes governing various processes. A major limitation with conventional gene expression microarray is that results obtained are biased due to gene probe design. The gene probes for expression microarrays are usually represented by a small number of probes located at the 3' end of a transcript. Tiling microarrays eliminate such issue by ...[more]

PMID: 22144238

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Whole transcription data for mouse spermatogonial cells

Project description:Spermatogenesis is a multi-step yet tightly regulated developmental process to produce male gemetes for reproduction. In mouse spermatogenesis, a sub-group of type A spermatogonia (Spga) known as spermatogonial stem cells (SSCs) maintain their population by self-renewal, and differentiate through mitosis and meiosis to form tetraploid (4n) pacchytene spermatocytes (Spcy) and haploid (n) round spermatids (Sptd), which further differentiate into functional sperms. In this study, we selected three representative stages of mouse spermatogenesis, namely Spga, Spcy and Sptd, to study their transcriptomic charteristics using whole genome tiling microarray.

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