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Transcription profile of SSEA-3-positive population of MSCs

ABSTRACT: Microarray analysis of the gene expression profile of SSEA-3-positive human adipose-derived MSCs were performed. Liposuction aspirates were obtained from the abdomen and thighs. Stromal vascular fraction (SVF) was isolated from the aspirated fat. Muse cells that express human SSEA-3 were collected from SVF using anti SSEA-3 antibody conjugated with PE and anti-PE microbeads. SSEA-3-negative fraction was considered M-bM-^@M-^XMuse-poor populationM-bM-^@M-^Y.

ORGANISM(S): Homo sapiens

SUBMITTER: Hisako Ishimine

PROVIDER: E-GEOD-55526 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Therapeutic Potential of Adipose-Derived SSEA-3-Positive Muse Cells for Treating Diabetic Skin Ulcers.

Kinoshita Kahori K Kuno Shinichiro S Ishimine Hisako H Aoi Noriyuki N Mineda Kazuhide K Kato Harunosuke H Doi Kentaro K Kanayama Koji K Feng Jingwei J Mashiko Takanobu T Kurisaki Akira A Yoshimura Kotaro K

Stem cells translational medicine 20150105 2

Stage-specific embryonic antigen-3 (SSEA-3)-positive multipotent mesenchymal cells (multilineage differentiating stress-enduring [Muse] cells) were isolated from cultured human adipose tissue-derived stem/stromal cells (hASCs) and characterized, and their therapeutic potential for treating diabetic skin ulcers was evaluated. Cultured hASCs were separated using magnetic-activated cell sorting into positive and negative fractions, a SSEA-3+ cell-enriched fraction (Muse-rich) and the remaining frac ...[more]

PMID: 25561682

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