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Genome-wide prediction of genes targeted by titrated levels of miR-17~92

ABSTRACT: The miR-17-92 cluster targets mRNAs involved in distinct pathways which either promote or inhibit tumor progression. However, the cellular and molecular mechanisms underlying miR-17~92 cluster mediated pro- or anti- tumorigenic effects has not been studied. In this study, we found that inhibition of colon cancer progression is dictated by quantitatively controlling expression of the miR-17~92 cluster. To identify molecular mechanisms giving rise to the different growth/metastasis patterns, we profiled gene expression in miR-Ctrl, miR-17~92Med and miR-17~92Hi cells using Affymetrix Murine GeneST Arrays. Total RNA from miR-Ctrl, miR-17~92Med and miR-17~92Hi cells (3 subclones of each group) was isolated and subsequently processed for microarray hybridization using Affymetrix.

ORGANISM(S): Mus musculus

SUBMITTER: Hong Jiang

PROVIDER: E-GEOD-55631 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The transcription factor TAL1 and the miR-17-92 cluster establish a regulatory loop

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