Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

SKIP Confers Salt Tolerance through Controlling Gene Alternative Splicing in Arabidopsis


ABSTRACT: A better understanding of the mechanisms for plant in response to abiotic stresses is key for the improvement of plant to resistant to the stresses. Much has been known for the regulation of gene expression in response to salt stress at transcriptional level, however, little is known at posttranscriptional level for this response. Recently, we identified that SKIP is a component of spliceosome and is necessary for the regulation of alternative splicing and mRNA maturation of clock genes. In this study, we observed that skip-1 is hypersensitive to salt stress. SKIP is necessary for the alternative splicing and mRNA maturation of several salt tolerance genes, e.g. NHX1, CBL1, P5CS1, RCI2A, and PAT10. Genome-wide analysis reveals that SKIP mediates the alternative splicing of many genes under salt stress condition, most of the new alternative splicing events in skip-1 is intron retention, which leads to the premature termination codon in their mRNA. SKIP also controls the alternative splicing by modulating the recognition or cleavage of 5' and 3' splice donor and acceptor sites under salt stress condition. Therefore, this study addresses a fundamental question on how the mRNA splicing machinery contributes to salt response at a posttranscriptional level. Totally six samples, two treatments and two genotypes, and each have two replicats.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Xu Gao 

PROVIDER: E-GEOD-55632 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

SKIP Confers Osmotic Tolerance during Salt Stress by Controlling Alternative Gene Splicing in Arabidopsis.

Feng Jinlin J   Li Jingjing J   Gao Zhaoxu Z   Lu Yaru Y   Yu Junya J   Zheng Qian Q   Yan Shuning S   Zhang Wenjiao W   He Hang H   Ma Ligeng L   Zhu Zhengge Z  

Molecular plant 20150121 7


Deciphering the mechanisms underlying plant responses to abiotic stress is key for improving plant stress resistance. Much is known about the regulation of gene expression in response to salt stress at the transcriptional level; however, little is known about this process at the posttranscriptional level. Recently, we demonstrated that SKIP is a component of spliceosome that interacts with clock gene pre-mRNAs and is essential for regulating their alternative splicing and mRNA maturation. In thi  ...[more]

Similar Datasets

2015-04-01 | GSE55632 | GEO
2012-12-13 | E-GEOD-32216 | biostudies-arrayexpress
2012-12-13 | GSE32216 | GEO
2023-02-02 | GSE144823 | GEO
2023-04-01 | GSE208786 | GEO
2023-09-06 | GSE222547 | GEO
| PRJNA240248 | ENA
2007-12-08 | GSE9734 | GEO
2023-12-08 | GSE229217 | GEO
2023-03-03 | PXD024816 | Pride