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Tead2 expression levels control the subcellular distribution of Yap and Taz, zyxin expression and epithelial-mesenchymal transition (ChIP-seq)

ABSTRACT: Cellular changes during an epithelial-mesenchymal transition (EMT) largely rely on global changes in gene expression orchestrated by transcription factors. Tead transcription factors and their co-factors Yap and Taz have been shown to be implicated in EMT, nevertheless, their direct target genes during EMT have remained elusive.We used genome-wide chromatin immunoprecipitation and next generation sequencing to identify diect Tead2 target genes during EMT. Py2T cells (murine breast cancer cell line) were treated with TGFβ for 5 days and subjected to ChIP using an antibody for Tead2 followed by next generation sequencing (Illumina HiSeq 2000; n=2)

ORGANISM(S): Mus musculus

SUBMITTER: Robert Ivanek

PROVIDER: E-GEOD-55709 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Tead2 expression levels control the subcellular distribution of Yap and Taz, zyxin expression and epithelial-mesenchymal transition.

Diepenbruck Maren M Waldmeier Lorenz L Ivanek Robert R Berninger Philipp P Arnold Phil P van Nimwegen Erik E Christofori Gerhard G

Journal of cell science 20140219 Pt 7

The cellular changes during an epithelial-mesenchymal transition (EMT) largely rely on global changes in gene expression orchestrated by transcription factors. Tead transcription factors and their transcriptional co-activators Yap and Taz have been previously implicated in promoting an EMT; however, their direct transcriptional target genes and their functional role during EMT have remained elusive. We have uncovered a previously unanticipated role of the transcription factor Tead2 during EMT. D ...[more]

PMID: 24554433

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