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Response of gcr1 null mutant to glucose

ABSTRACT: This experiment was done to detect the transcriptomic defects in the gcr1 null mutant, relative to an isogenic wild type, in the immediate response to glucose. Keywords: time course Both gcr1 mutant and wild type cells were grown in YEP containing 3% pyruvate to early/mid log phase. The 0min sample was taken just prior to glucose addition. After addition of glucose, samples were taken at 20min and 60min. Total RNA was extracted for both strains and expression was measured by competitive hybridization of the mutant and wild type labeled cRNA for each time point.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Kellie Barbara

PROVIDER: E-GEOD-5575 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The transcription factor Gcr1 stimulates cell growth by participating in nutrient-responsive gene expression on a global level.

Barbara Kellie E KE Haley Terry M TM Willis Kristine A KA Santangelo George M GM

Molecular genetics and genomics : MGG 20061124 2

Transcriptomic reprogramming is critical to the coordination between growth and cell cycle progression in response to changing extracellular conditions. In Saccharomyces cerevisiae, the transcription factor Gcr1 contributes to this coordination by supporting maximum expression of G1 cyclins in addition to regulating both glucose-induced and glucose-repressed genes. We report here the comprehensive genome-wide expression profiling of gcr1Delta cells. Our data show that reduced expression of ribos ...[more]

PMID: 17124610

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