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Profiling of copy-number variation in tumors from heterozygous and homozygous Tp53C273X knockout rats

ABSTRACT: We used Nimblegen HD aCGH to detect copy-number variants between tumor and ear control-DNA samples from heterozygous and homozygous Tp53C273X knockout rats Comparison of tumor DNA with ear control-DNA from the same animal

ORGANISM(S): Rattus norvegicus

SUBMITTER: Roel Hermsen

PROVIDER: E-GEOD-55895 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Lack of major genome instability in tumors of p53 null rats.

Hermsen Roel R Toonen Pim P Kuijk Ewart E Youssef Sameh A SA Kuiper Raoul R van Heesch Sebastiaan S de Bruin Alain A Cuppen Edwin E Simonis Marieke M

PloS one 20150326 3

Tumorigenesis is often associated with loss of tumor suppressor genes (such as TP53), genomic instability and telomere lengthening. Previously, we generated and characterized a rat p53 knockout model in which the homozygous rats predominantly develop hemangiosarcomas whereas the heterozygous rats mainly develop osteosarcomas. Using genome-wide analyses, we find that the tumors that arise in the heterozygous and homozygous Tp53C273X mutant animals are also different in their genomic instability p ...[more]

PMID: 25811670

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