Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Gene Expression Profiling of Prostate Benign and Malignant Tissue


ABSTRACT: We profiled genome-wide gene expression of human prostate benign and malignant tissue to identify potential biomarkers and immunotherapy targets. We stratified malignant specimens according to their TMPRSS2:ERG gene fusion status. Radical prostatectomy tissue samples were obtained from the Hershey Foundation Prostate Cancer Serum and Tumor Bank at our institution. Morphologic diagnosis was done by a pathologist. OCT blocks containing >30% of PCa tissue (with Gleason score of 6 or 7) were selected for RNA purification. A biopsy punch was used to select the PCa tissues from the OCT sample blocks. Benign or PCa tissues were homogenized using a TissueLyser (Qiagen) at 28 Hz for 5 min. Total RNA was isolated using Trizol reagent. RNA was quantified by NanoDrop ND-1000 spectrophotometer, and quality was evaluated with Agilent RNA 6000 NanoChip and the 2100 Bioanalyzer, with 28S/18S ratios and RIN determined by 2100 Expert software.

ORGANISM(S): Homo sapiens

SUBMITTER: M. Simo Arredouani 

PROVIDER: E-GEOD-55945 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

Identification of the transcription factor single-minded homologue 2 as a potential biomarker and immunotherapy target in prostate cancer.

Arredouani Mohamed S MS   Lu Bin B   Bhasin Manoj M   Eljanne Miriam M   Yue Wen W   Mosquera Juan-Miguel JM   Bubley Glenn J GJ   Li Vivian V   Rubin Mark A MA   Libermann Towia A TA   Sanda Martin G MG  

Clinical cancer research : an official journal of the American Association for Cancer Research 20090908 18


<h4>Purpose</h4>Identification of novel biomarkers and immunotherapy targets for prostate cancer (PCa) is crucial to better diagnosis and therapy. We sought to identify novel PCa tumor-associated antigens (TAA) that are expressed in PCa, absent in nonprostate human tissue, and immunogenic for immune responses restricted by human HLA.<h4>Experimental design and results</h4>Using microarray analysis of normal and cancerous human prostate tissues, we identified 1,063 genes overexpressed in PCa. Aft  ...[more]

Similar Datasets

2013-09-27 | E-GEOD-49298 | biostudies-arrayexpress
2013-08-02 | E-GEOD-47973 | biostudies-arrayexpress
2012-07-12 | E-GEOD-39314 | biostudies-arrayexpress
2008-12-01 | GSE9972 | GEO
2021-03-07 | PXD019443 | Pride
2022-10-25 | PXD037031 | Pride
2013-11-15 | E-GEOD-48430 | biostudies-arrayexpress
2012-12-27 | E-GEOD-43146 | biostudies-arrayexpress
2021-09-08 | PXD014291 | Pride
2008-12-01 | E-GEOD-9972 | biostudies-arrayexpress