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Comparative genomic hybridization of CHO Chr6 cell line

ABSTRACT: Aim is to validate content of human genomic material in CHO cells monosomal for human chromosome 6. Comparative genomic hybridization to define human genomic regions maintained in hamster hybridoma cell line.

ORGANISM(S): Cricetulus griseus

SUBMITTER: Claus Scholz

PROVIDER: E-GEOD-56334 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Vγ9Vδ2 TCR-activation by phosphorylated antigens requires butyrophilin 3 A1 (BTN3A1) and additional genes on human chromosome 6.

Riaño Felipe F Karunakaran Mohindar M MM Starick Lisa L Li Jianqiang J Scholz Claus J CJ Kunzmann Volker V Olive Daniel D Amslinger Sabine S Herrmann Thomas T

European journal of immunology 20140630 9

Pyrophosphorylated metabolites of isoprenoid-biosynthesis (phosphoantigens, PAgs) activate Vγ9Vδ2 T cells during infections and trigger antitumor activity. This activation depends on expression of butyrophilin 3 A1 (BTN3A1) by antigen-presenting cells. This report defines the minimal genetic requirements for activation of Vγ9Vδ2 T cells by PAgs and mAb 20.1. We compared PAg-presentation by BTN3A1-transduced CHO hamster cells with that of CHO cells containing the complete human chromosome 6 (Chr6 ...[more]

PMID: 24890657

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