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Transcription profiling of human MDA-MB-435 Cells treated with 2-methoxyestradiol versus control DMSO

ABSTRACT: MDA-MB-435 breast cancer cells were treated with 2ME2 (2 ÂµM) or vehicle alone. RNA was extracted and genomic profiling was performed using 22k Agilent microarrays. Experiment Overall Design: MDA-MB-435 breast cancer cells were treated with 2ME2 (2 ÂµM) or vehicle alone. RNA was extracted and genomic profiling was performed using 22k Agilent microarrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Nancy DeMore

PROVIDER: E-GEOD-5665 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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MDA-MB-435 Cells treated with 2-methoxyestradiol versus control DMSO

Project description:MDA-MB-435 breast cancer cells were treated with 2ME2 (2 µM) or vehicle alone. RNA was extracted and genomic profiling was performed using 22k Agilent microarrays. Keywords: Treatment-response

2006-09-08 | GSE5665 | GEO

Next Generation Sequencing of MCF-7 and MDA-MB-435 cells

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aTEA 40 uM 12 hour treatment for MDA-MB-435 cells

Project description:In an effort to identify novel signaling molecules modulated by α-TEA, microarray analyses were performed. DNA array data obtained from human MDA-MB-435 breast cancer cells treated with 40 µM α-TEA for 12 h revealed over 400 genes that were consistently either up- or down-regulated. Thirty-four genes were selected based on their possible involvement in the known biological activities of α-TEA, and three: phorbol-12-myristate 13-acetate-induced protein (PMAPI) which codes for the protein NOXA, ABL2 which codes for the protein referred to as ARG (Abelson-related gene) and THBS1 which codes for thrombospondin 1, TSP-1 were chosen for further study. Keywords: Anti-cancer drug (aTEA at 40 uM 12 hour) treatment for MDA-MB-435 cells

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Next Generation Sequencing of MCF-7 and MDA-MB-435 cells

Project description:Human breast cancer cells MDA-MB-435 had higher metastatic potential and other aggressive characteristics than MCF-7 cells. Next-generation RNA sequencing (RNA-Seq) was used to clear this concern through comparison the transcriptomic expression profiles of these two cells. Total RNA were extracted from MCF-7 and MDA-MB-435S cells, and the polyA+ mRNA was sequenced using Illumina Genome Analyzer IIx. The reads were mapped to RefSeq RNA reference sequences.

2014-01-17 | E-GEOD-54122 | biostudies-arrayexpress

Breast cancer MCF-7 Cells and MDA-MB-435 cells: pre-miR-125b Transfected cells vs. pre-control transfected cells

Project description:Transcriptional profiling of breast cancer cells comparing pre-control transfected cells with cells transfected with pre-miR-125b. We searched for miR-125b targets by systematic screening of mRNA profiling of pre-miR-125b transfected MCF-7 cells and MDA-MB-435 cells.

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Transcription profiling of human MDA-MB-435 breast carcinoma cell line transfected with the integrin beta4 subunit revealsIntegrin alpha6-beta4 control the expression of genes associated with cell motility, invasion and metastasis

Project description:The objective of this study was to determine the gene expression changes mediated by the alpha6beta4 integrin using MDA-MB-435 breast carcinoma cell line under normal culturing conditions (10% FCS in DMEM). Experiment Overall Design: Comparison of clones of MDA-MB-435 cells transfected with the integrin beta4 subunit (which results in cell surface expression of the integrin apha6beta4 integrin; clones 3A7 and 5B3) to those transfected with vector (mock; 6D2 and 6D7) only.

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Breast cancer MCF-7 Cells and MDA-MB-435 cells: pre-miR-125b Transfected cells vs. pre-control transfected cells

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Minocycline is an inhibitor of the TNF-alpha induced fusion of human M13SV1-Cre breast epithelial cells and human MDA-MB-435-pFDR1 breast cancer cells

Project description:Using a Cre-LoxP-based cell fusion assay we able to demonstrate that the fusion of human M13SV1-Cre breast epithelial cells and human MDA-MB-435-pFDR1 breast cancer cells was induced by TNF-alpha both under normoxic and hypoxic conditions. To investigate the impact of TNF-alpha on the gene expression profile of the cells M13SV1 and MDA-MB-435-pFDR1 cells were cultured for three days in the presence of 100ng/ml TNF-alpha both under normoxic and hypoxic conditions. Untreated cells served as a control. Subsequently, total RNA was extracted from the cells. Total RNA of three independent experiments that matched RIN criteria of 8 to 10 was pooled. Synthesis and Cy3 labeling of cDNA and hybridization of microarrays and microarray analysis was performed by Source Biosciences (Nottingham, UK). Microarray gene expression data were analyzed using GeneSpring GX v14.8 software (Agilent Technologies).

2018-04-23 | E-MTAB-6084 | biostudies-arrayexpress

CPYPP induces paraptosis in MDA-MB-231 and MDA-MB-435 melanoma cells

Project description:CPYPP induces paraptosis in MDA-MB-231 and MDA-MB-435 melanoma cells

| PRJNA1092744 | ENA

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Project description:This experiment is designed to screen miRNAs that are deregulated during breast cancer metastasis. Comparatively analyzing miRNAs in parental MDA-MB-435 cells and cells obtained from its lung metastases, 23 miRNAs expressed differentially, among which 12 were elevated and 11 were down-regulated. Total RNA were extracted from parental MDA-MB-435 cells and cells obtained from its lung metastases after 30 days inoculation. Two biological replications for each treatment.

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