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Expression analysis of genes in Rattus norvegicus lungs at postnatal day 4, 10 and 21 after intrauterine growth restriction induced by low protein diet

ABSTRACT: We performed RNA microarray in a low protein diet (LPD) model of IUGR at three key time points of alveolarization process. IUGR and control rat pups had been studied for each time point considered: on postnatal day 4 (P4) before beginning of the alveolarization process, on P10, peak of alveolarization process and on P21 at the end of it. A twelve chip study using total RNA extracted from 14 whole lungs intrauterine growth restricted rat pups compared to 15 controls. RNA was extracted at three key time points of alveolarization in postnatal life. RNA was pooled by groups of 2 ou 3 before analysis.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Elodie Zana-TaM-CM-/eb

PROVIDER: E-GEOD-56956 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Impaired alveolarization and intra-uterine growth restriction in rats: a postnatal genome-wide analysis.

Zana-Taieb E E Pham H H Franco-Montoya M L ML Jacques S S Letourneur F F Baud O O Jarreau P H PH Vaiman D D

The Journal of pathology 20150201 3

Intra-uterine growth restriction (IUGR) dramatically increases the risk of bronchopulmonary dysplasia in preterm babies, a disease characterized by arrested alveolarization and abnormal microvascular angiogenesis. We have previously described a rodent low protein diet (LPD) model of IUGR inducing impaired alveolarization, but failed to demonstrate any modification of the classical factors involved in lung development. We performed a genome-wide microarray analysis in 120 rat pups with LPD-induce ...[more]

PMID: 25347958

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