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Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from melanoma xenograft tumors originating from primary immortalized melanocytes

ABSTRACT: PREX2 truncating mutations occur in melanoma. We used microarray based gene expression profiling to compare expression patterns between xenografts harboring control GFP, wt PREX2 or various human relevant PREX2 mutants Primary xenograft tumors derived from primary immortalized melanocytes expressing indicated constructs were grown in ncr-nude mice and tumors harvested before before reaching 1.5cm size

ORGANISM(S): Homo sapiens

SUBMITTER: Lynda Chin

PROVIDER: E-GEOD-57309 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Truncating PREX2 mutations activate its GEF activity and alter gene expression regulation in NRAS-mutant melanoma.

Lissanu Deribe Yonathan Y Shi Yanxia Y Rai Kunal K Nezi Luigi L Amin Samir B SB Wu Chia-Chin CC Akdemir Kadir C KC Mahdavi Mozhdeh M Peng Qian Q Chang Qing Edward QE Hornigold Kirsti K Arold Stefan T ST Welch Heidi C E HC Garraway Levi A LA Chin Lynda L

Proceedings of the National Academy of Sciences of the United States of America 20160216 9

PREX2 (phosphatidylinositol-3,4,5-triphosphate-dependent Rac-exchange factor 2) is a PTEN (phosphatase and tensin homolog deleted on chromosome 10) binding protein that is significantly mutated in cutaneous melanoma and pancreatic ductal adenocarcinoma. Here, genetic and biochemical analyses were conducted to elucidate the nature and mechanistic basis of PREX2 mutation in melanoma development. By generating an inducible transgenic mouse model we showed an oncogenic role for a truncating PREX2 mu ...[more]

PMID: 26884185

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