Unknown,Transcriptomics,Genomics,Proteomics

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Novel culture conditions facilitating the induction of naïve pluripotency in human embryonic stem cells


ABSTRACT: We report a novel culture condition inducing naive pluripotency in hESCs in a rapid, robust and reproducible way. These naive hESCs were similar to mESCs exhibiting domed colony morphology, increased single survival, reduced doubling time, upregulation of naive pluripotency-specific genes, unbiased lineage-specific differentiation, hypomethylation, separate clustering profile from parental primed hESCs and were dependent on signalling pathways similar to naive mESCs. Global DNA methylation analysis of naive hESCs and their parental primed hESC counterparts.

ORGANISM(S): Homo sapiens

SUBMITTER: Matthias Roost 

PROVIDER: E-GEOD-57318 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Human embryonic stem cells (hESCs) closely resemble mouse epiblast stem cells exhibiting primed pluripotency unlike mouse ESCs (mESCs), which acquire a naïve pluripotent state. Efforts have been made to trigger naïve pluripotency in hESCs for subsequent unbiased lineage-specific differentiation, a common conundrum faced by primed pluripotent hESCs due to heterogeneity in gene expression existing within and between hESC lines. This required either ectopic expression of naïve genes such as NANOG a  ...[more]

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