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RNAi knock down of Arabidopsis polycomb binding protein

ABSTRACT: We have identified a gene encoding a polycomb-chromo domain binding protein. Complete knockout of these plants do not lead to a phenotype, but a reduction of gene expression in RNAi line produces a defined phenotype. This argues in favour of a compensation mechanism that is switched on once the concentration of the gene reaches a control level.The aim of the experiment is to search for such compensatory factors that are up-regulated in the knockout line. Experimenter name = Katharine Cain; Experimenter phone = 01133433146; Experimenter department = University of Leeds; Experimenter address = University of Leeds; Experimenter address = Clarendon Way; Experimenter address = Leeds; Experimenter zip/postal_code = LS29JT; Experimenter country = UK Experiment Overall Design: 4 samples were used in this experiment

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Nottingham Arabidopsis Stock Centre (NASC)

PROVIDER: E-GEOD-5740 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Polycomb Binding Protein

Project description:We have identified a gene encoding apolycomb-chromo domain binding protein. Complete knockout of these plants do not lead to a phenotype, but a reduction of gene expression in RNAi line produces a defined phenotype. This argues in favour of a compensation mechanism that is switched on once the concentration of the gene reaches a control level.The aim of the experiment is to search for such compensatory factors that are up-regulated in the knockout line. Experimenter name = Katharine Cain Experimenter phone = 01133433146 Experimenter department = University of Leeds Experimenter address = University of Leeds Experimenter address = Clarendon Way Experimenter address = Leeds Experimenter zip/postal_code = LS29JT Experimenter country = UK Keywords: genetic_modification_design

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2007-01-22 | GSE5749 | GEO

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