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Transcription profiling of Arabidopsis exposed to red light illumination with and without lincomycin

ABSTRACT: To identify putative regulatory elements enriched in the promoters of target genes of the PGE-dependent retrograde signaling pathway, we analyzed 500 bp regions of sequence upstream of genes whose expression was down-regulated by lincomycin. We treated dark-grown Arabidopsis seedlings with lincomycin, sampled them before or after a short illumination and examined the genomic response using Affymetrix ATH1 oligonucleotide microarrays. Differentially regulated genes were ranked based on descending degree of significance (p-value) and the top 50 genes affected by lincomycin in dark grown seedlings and top 50 genes affected by the antibiotic after illumination were selected for further analysis. Experiment Overall Design: 2*2 factorial design, two variables are (1) with/without lincomycin treatment (2) with/without red illumination. There are 2 replicates for each condition.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Fangxin Hong

PROVIDER: E-GEOD-5759 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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