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Next-Generation Sequencing of Small RNAs from HIV-Infected Cells Identifies Phased microRNA Expression Patterns and Candidate Novel microRNAs Differentially Expressed upon Infection

ABSTRACT: HIV infection of CD4 T cells induces a range of host transcriptional changes in mRNAs as well as microRNAs that may coordinate changes in mRNAs. We applied next-generation sequencing, analyzing the small RNA fraction of HIV-infected cells at 5, 12, and 24 h postinfection (RNA-Seq).

ORGANISM(S): Homo sapiens

SUBMITTER: Michael Katze

PROVIDER: E-GEOD-57763 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Next-generation sequencing of small RNAs from HIV-infected cells identifies phased microrna expression patterns and candidate novel microRNAs differentially expressed upon infection.

Chang Stewart T ST Thomas Matthew J MJ Sova Pavel P Green Richard R RR Palermo Robert E RE Katze Michael G MG

mBio 20130205 1

HIV infection of CD4(+) T cells induces a range of host transcriptional changes in mRNAs as well as microRNAs that may coordinate changes in mRNAs. To survey these dynamic changes, we applied next-generation sequencing, analyzing the small RNA fraction of HIV-infected cells at 5, 12, and 24 h postinfection (RNA-Seq). These time points afforded a view of the transcriptomic changes occurring both before and during viral replication. In the resulting small RNA-Seq data set, we detected a phased pat ...[more]

PMID: 23386435

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