Project description:miRNA expression was determined using the Agilent Human miRNA Microarray G4870A (miRNA ID version) One-channel experiment: Control cells, 2.5%FCS, 2.5% FCS + Dex; Biological replicates: 4
Project description:mRNA expression was determined using the Agilent-G4851A SurePrint G3 Human GE 8x60K Microarray Three-condition experiment, Control vs. FCS vs. Dex+FCS stim cells. Biological replicates: 4
Project description:This SuperSeries is composed of the following subset Series: GSE37758: Aspergillus niger : Control (fructose) vs. steam-exploded sugarcane induction (SEB) GSE37760: Aspergillus niger : Control (fructose) vs. xylose + arabinose (XA) Refer to individual Series
Project description:Transcriptional profiling of A. niger comparing mutant strains with the disrupted xylanolytic transcriptional activator gene, XlnR, the arabinolytic transcriptional activator gene, AraR, and the double mutant (M-NM-^TXlnR, M-NM-^TaraR and M-NM-^TaraRM-NM-^TXlnR, repsectively) treated with steam-exploded sugarcane bagasse (SEB) for 12 and 24 h. The main objective was to identify genes related to cellulases and hemicellulases in mutant strains grown on SEB, with indirect comparisons with the WT strain grown on SEB [the (WT/SEB) data deposited in GSE24798]. The experiment was further validated by real-time PCR and enzymatic assay. Two-condition experiment : A. niger mutant strains on SEB for 12 and 24 h at 30 oC in batch culture. Firstly, the strains were pre-grown in minimal medium with fructose as carbon source (control), and then transferred to SEB as carbon source.
Project description:Transcriptional profiling of A. niger comparing different strains (WT, M-NM-^TXlnR, M-NM-^TaraR and M-NM-^TaraRM-NM-^TXlnR) treated with 25mM xylose + 25mM arabinose for 2 and 8h. The main objective was to identifiy genes related to primary metabolism and carbohydrate metabolism in general, for instance, cellulases and hemicellulases. The study will compare the differences between WT strain and the strains with the disrupted xylanolytic transcriptional activator gene, XlnR, the arabinolytic transcriptional activator gene, AraR, and the double mutant,after treatment with xylose+arabinose (XA treatment). The experiment was further validated by real-time PCR and enzymatic assays. Two-condition experiment : A. niger mutant strains on XA for 2 and 8 h at 30 oC in batch culture. Firstly, the strains were pre-grown in minimal medium with fructose as carbon source (control), and then transferred to xylose+arabinose (25 mM each) as carbon source.
Project description:Transcriptional profiling of A. nidulans comparing the mutant strain alcApkcA M-NM-^TcnaA and M-NM-^TcnaA grown on alcA inducing conditions. The main objective was to identifiy genes related to M-NM-^TcnaA phenotype supression by pkcA overexpression. The experiment was further validated by real-time PCR. Two-condition experiment : A. nidulans strains grown during 24 and 48 h at 37oC in glycerol 2% threonine 100mM.
Project description:Transcriptional profiling of A. nidulans comparing the mutant strains M-NM-^TgprB and M-NM-^TgprD grown on 1% glucose (w/v) in a batch cultivation medium (BCM) at 37M-BM-0C for 24 and 48h. The main objective was to identifiy genes related to gprB and gprD gene function. The experiment was further validated by real-time PCR and enzymatic assay. Two-condition experiment : A. nidulans strains grown during 24 and 48 h at 37M-BM-0C in glucose 1% (w/v).
Project description:The main objective was to identifiy genes related to scrA- extragenic suppression of crzA. Thus, through transcriptional profiling, we characterised the transcriptional basis of calcium tolerance in our mutant strain, M-NM-^TcrzA/scrA-. To evaluate the effect of calcium on global A. nidulans gene expression, we performed competitive microarray hybridizations using RNA obtained from the wild-type, M-NM-^TcrzA and M-NM-^TcrzA/scrA- strains before and after short pulses (10 and 30 minutes) of 200 mM calcium chloride. The experiment was further validated by real-time PCR. Two-condition experiment : A. nidulans wild-type, M-NM-^TcrzA and M-NM-^TcrzA/scrA- strains with 200 mM CaCl2 for 10 and 30 min at 30M-BM-0C and 150 rpm in 25 mL YAG medium. Firstly, the strains were grown overnight at 30M-BM-0C and 150 rpm in YAG medium (preculture). Afterwards, the medium was switched by a new media (25 mL) plus calcium and incubated as described above. Control condition refers to the preculture in YAG medium without further incubation.
Project description:Transcriptional profiling of Acinetobacter baumannii ATCC17978 cells comparing treated ethanol cells with oleanolic acid treated. Based on the gene expression, we performed experiments to confirm the therapeutic effect and mechanism of OA in A. baumannii. We performed a transcriptome anaylsis of 2 samples that are OA and ethanol treatment, respectively.
Project description:Transcriptional profiling of A. nidulans comparing Xylose and Fructose grown on Wild type strain. The main objective was to identifiy genes related to Xylose transport. The experiment was further validated by real-time PCR. Three-condition experiment : A. nidulans strains grown during 16 h on fructose and transfered to xylose for 6, 12 and 24h.