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Unknown,Transcriptomics,Genomics,Proteomics

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4TO7 cells: miR-23b/27b/24 overexpression vs control

ABSTRACT: Transcriptional profiling of 4TO7 cells featuring ectopic expression of miR-23b/27b/24 compared to vector control cells. Gene expression changes will be examined as potential direct targets of the miRNAs for use in later studies on metastasis suppression or activation. Two-condition experiment, miRNA overexpressing cells vs vector control. Biological replicates: 2 transfection replicates each.

ORGANISM(S): Mus musculus

SUBMITTER: Yong Wei

PROVIDER: E-GEOD-57838 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The microRNA-23b/27b/24 cluster promotes breast cancer lung metastasis by targeting metastasis-suppressive gene prosaposin.

Ell Brian B Qiu Qiong Q Wei Yong Y Mercatali Laura L Ibrahim Toni T Amadori Dino D Kang Yibin Y

The Journal of biological chemistry 20140625 32

MicroRNAs (miRNAs) have been shown to function as key regulators of tumor progression and metastasis. Recent studies have indicated that the miRNAs comprising the miR-23b/27b/24 cluster might influence tumor metastasis, although the precise nature of this regulation remains unclear. Here, expression of the miR-23b/27b/24 cluster is found to correlate with metastatic potential in mouse and human breast cancer cell lines and is elevated in metastatic lung lesions in human breast cancer patients. E ...[more]

PMID: 24966325

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4TO7 cells: miR-23b/27b/24 overexpression vs control

Project description:Transcriptional profiling of 4TO7 cells featuring ectopic expression of miR-23b/27b/24 compared to vector control cells. Gene expression changes will be examined as potential direct targets of the miRNAs for use in later studies on metastasis suppression or activation.

2014-12-23 | GSE57838 | GEO

MiR-23b/-27b Targets in Prostate Cancer Cell Lines

2016-02-10 | GSE76620 | GEO

MiR-23b/-27b Targets in Prostate Cancer Cell Lines

Project description:Analysis of genes regulated by miR-23b/-27b overexpression in aggressive PC3-ML cells, confirmed by antagomiR inhibition of miR-23b and miR-27b in the relatively indolent cell line LNCaP. Genes that were downregulated in PC3-ML overexpression and upregulated with LNCaP inhibition were further explored as downstream targets of miR-23b/-27b. PC3-Ml cells were transduced with miR-23b/-27b or a scrambled miRNA control, and only cells expressing greater than 95% transduction efficiency were used for array. LNCaP cells were transfected with antagomiRs to miR-23b and miR-27b, or a non-coding control.

2016-02-10 | E-GEOD-76620 | biostudies-arrayexpress

Dysregulation of a miR-23b/27b-p53 axis impairs muscle differentiation in humans with type 2 diabetes

Project description:Impaired skeletal muscle function is a central feature in the pathophysiology of type 2 diabetes (T2DM). The disease phenotype could be due to immature muscle cell development, which in turn may occur as the result of disturbed microRNA-mediated regulation of muscle differentiation in T2DM. To address this hypothesis, we assessed global miRNA expression during in vitro differentiation of muscle stem cells derived from T2DM patients and healthy controls. We identified the mir-23b/27b cluster to be downregulated in the patients, and further demonstrated that a pro-myogenic effect of these miRNAs occurs through targeting of several genes in the p53 pathway, which was concordantly dysregulated in the muscle cells derived from humans with T2DM. In conclusion, we have identified a novel myogenesis-controlling pathway, the miR-23b/27b-p53 axis, potentially contributing to the sustained multiple muscular dysfunctions in T2DM in humans.

2017-08-12 | GSE86069 | GEO

Mus musculus

Project description:4TO7 cells: miR-23b/27b/24 overexpression vs control

| PRJNA248212 | ENA

Identification of microRNA-27b target genes in breast cancer cell lines

Project description:To identify the microRNA-27b (miR-27b) target genes in luminal-type breast cancer cells, we performed the microarray analysis using miR-27b knockdown MCF7-luc cell line (MCF7-luc anti-miR-27b), miR-27b overexpressing MCF7-luc cell line (MCF7-luc miR-27b o.e.) and their contro cell line (MCF7-luc anti-NC). After establishing MCF7-luc anti-miR-27b, MCF7-luc miR-27b o.e. and MCF7-luc anti-NC using lentivirus vector, we performed the microarray analysis.

2015-06-18 | E-GEOD-67631 | biostudies-arrayexpress

RNA-seq in breast cancer cell lines after manipulation of miR-23b expression

Project description:RNA sequencing technology has been carried out in order to evaluate mRNA expression changes after manipulation of miR-23b in both MCF-7 and MDA-MB-231 breast cancer cell lines Our study implicates miR-23b in cytoskeletal remodeling in breast cancer. To evaluate the entire set of genes modulated by miR-23b we performed RNA-seq after ectopic manipulation of this miRNA in breast cancer cell lines. We over-expressed this miRNA in MCF-7 epithelial cancer cell lines and also reduced its activity by stably transfecting MDA-MB-231 mesenchymal-like cancer cell lines with a specific sponge vector. RNA-seq analysis revealed a number of candidate targets of this miRNA.

2013-05-01 | E-GEOD-37918 | biostudies-arrayexpress

miR-27b synergizes with anticancer drugs

Project description:Drug resistance, caused by complex and redundant mechanisms, is a major obstacle in cancer treatment, especially in liver and kidney cancers. Combinational therapy of miRNAs, which concurrently target multiple pathways, with anticancer drugs represent a new strategy to improve the drug response. By a systems approach, we identified that miR-27b, a miRNA deleted in liver and kidney cancers, sensitizes cancer cells to a broad spectrum of anticancer drugs in vitro and in vivo. Two samples transfected with nontarget miRNA control or miR-27b mimics followed by 48 hours doxorubicin treatment

2014-01-07 | E-GEOD-53872 | biostudies-arrayexpress

Identification of microRNA-27b target genes in breast cancer cell lines

2015-06-18 | GSE67631 | GEO

The effect of knocking down miR-27b on DIV14 mouse cortical neurons

Project description:To understand the effect of reducing miR-27b levels on genome-wide expression in cortical neurons and identify potential targets of miR-27b, we generated dissociated cortical neuron cultures and treated them with lentivirus to knock down miR-27b. 2 treatment conditions (control lentivirus or lentivirus to knock down miR-27b); 3 neuronal preparations of dissociated mouse cortical neurons; total of 6 samples

2015-09-22 | E-GEOD-73275 | biostudies-arrayexpress

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