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Transcription profiling of human cervical cancer patient biopsies to explore intratumour heterogeneity reveals that intratumor variation is low for most probe sets tested but that multiple biopsies are recommended for reliable estimates of gene expression

ABSTRACT: To explore intratumor heterogeneity in gene expression profiles from patients with cervical cancer. Experimental Design: A total of 33 biopsies were obtained from 11 patients, sampling between two and five different areas for each tumor.The extracted RNA was hybridized onto the Affymetrix U133 Plus 2.0 oligonucleotide chip. The variance of expression within a patient (W), between patients (B) and the total variance (T =W + B) were calculated for each ProbeSet, and the ratio W/T was used as a measure of intratumor heterogeneity. Gene Ontology functional analysis was done to assess the function of genes that had high W/T (top 10%) and low W/T (bottom 10%) values.

ORGANISM(S): Homo sapiens

SUBMITTER: Paul Boutros 

PROVIDER: E-GEOD-5787 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression profiling in cervical cancer: an exploration of intratumor heterogeneity.

Bachtiary Barbara B   Boutros Paul C PC   Pintilie Melania M   Shi Willa W   Bastianutto Carlo C   Li Jian-Hua JH   Schwock Joerg J   Zhang Wendy W   Penn Linda Z LZ   Jurisica Igor I   Fyles Anthony A   Liu Fei-Fei FF  

Clinical cancer research : an official journal of the American Association for Cancer Research 20061001 19

<h4>Purpose</h4>To explore intratumor heterogeneity in gene expression profiles from patients with cervical cancer.<h4>Experimental design</h4>A total of 33 biopsies were obtained from 11 patients, sampling between two and five different areas for each tumor. The extracted RNA was hybridized onto the Affymetrix U133 Plus 2.0 oligonucleotide chip. The variance of expression within a patient (W), between patients (B) and the total variance (T = W + B) were calculated for each ProbeSet, and the rat  ...[more]

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