Transcription profiling of human prolymphocytic leukemina T-cells and normal T-cells

ABSTRACT: T-cell prolymphocytic leukemina (T-PLL) is an agressive lymphoma derived from mature T-cells, which is in most cases characterized by the presence of an inv(14)(q11q32) and a characteristic pattern of secondary chromosomal abberations. We used microarrays to compare the transcriptomes of eight immunomagnetically purified CD3+ normal donor derived peripheral blood cells with five highly purified inv(14)-positive T-PLL blood samples. Experiment Overall Design: Purified T-PLL cells and normal T-cells were analyzed on microarrays to identify differentially expressed genes. High-resolution copy number determination using SNP-chip technology and FISH in twelve inv(14)-positive T-PLL showed that differentially expressed genes clustered significantly in regions affectd by recurrent chromosomal imbalances.

ORGANISM(S): Homo sapiens

SUBMITTER: Ludger Klein-Hitpass 

PROVIDER: E-GEOD-5788 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress