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ChIP-exo analysis of the DNA-binding sites of the yeast transcription factor Yfl052w sequenced by Illumina

ABSTRACT: We determined DNA-binding sites of the yeast transcription factor Yfl052w by ChIP-exo. Cells were grown in the YP media containing palatinose. Yfl052w was tagged with HA tag and anti-HA antibody was used for the immunoprecipitation. Examination of Yfl052 trancription factor in HA-tagged and wt cells (as a control)

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Ksenia Pougach

PROVIDER: E-GEOD-57900 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Duplication of a promiscuous transcription factor drives the emergence of a new regulatory network.

Pougach Ksenia K Voet Arnout A Kondrashov Fyodor A FA Voordeckers Karin K Christiaens Joaquin F JF Baying Bianka B Benes Vladimir V Sakai Ryo R Aerts Jan J Zhu Bo B Van Dijck Patrick P Verstrepen Kevin J KJ

Nature communications 20140910

The emergence of new genes throughout evolution requires rewiring and extension of regulatory networks. However, the molecular details of how the transcriptional regulation of new gene copies evolves remain largely unexplored. Here we show how duplication of a transcription factor gene allowed the emergence of two independent regulatory circuits. Interestingly, the ancestral transcription factor was promiscuous and could bind different motifs in its target promoters. After duplication, one paral ...[more]

PMID: 25204769

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ChIP-exo analysis of the DNA-binding sites of the yeast transcription factor Yfl052w sequenced by SOLiD

Project description:We determined DNA-binding sites of the yeast transcription factor Yfl052w by ChIP-exo. Cells were grown in the YP media containing palatinose. Yfl052w was tagged with HA tag and anti-HA antibody was used for the immunoprecipitation. Examination of Yfl052 trancription factor in HA-tagged and wt cells (as a control)

2014-07-24 | E-GEOD-57901 | biostudies-arrayexpress

ChIP-exo analysis of the DNA-binding sites of the yeast transcription factor Yfl052w

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Project description:Mapping ultra high resolution of Brachyury:DNA interaction would provide us with valuable new mechanistic insights into complex molecular transactions at Brachyury-bound enhancers. Embryonic stem cells were differentiated into Brachyury-positive mesoendoderm cells. And, ChIP-exo experiment was then performed to identify detailed Brachyury-DNA binding profiles.

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