Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

48 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Analysis of aortic gene expression in ovariectomized ApoE-deficient mice substituted with medroxyprogesterone acetate, norethisterone acetate or placebo

ABSTRACT: Agilent 4x44k Whole Mouse Genome v1 Microarrays were used to analyze aortic transcriptome of mice substituted with medroxyprogesterone acetate, norethisterone acetate or placebo after having been ovariectomized. The aim of this experiment was to detect genes regulated in the gestagen-treated groups as compared to placebo that might be involved in thrombotic events Aortic gene expression was analyzed in ApoE-deficient mice after ovariectomy and 90 days of hormone- or placebo-treatment (medroxyprogesterone acetate or norethisterone acetate) and feeding a high-fat Western-type diet.

ORGANISM(S): Mus musculus

SUBMITTER: Rene Deenen

PROVIDER: E-GEOD-57972 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Analysis of aortic gene expression in ovariectomized ApoE-deficient mice substituted with medroxyprogesterone acetate, norethisterone acetate or placebo

Project description:Agilent 4x44k Whole Mouse Genome v1 Microarrays were used to analyze aortic transcriptome of mice substituted with medroxyprogesterone acetate, norethisterone acetate or placebo after having been ovariectomized. The aim of this experiment was to detect genes regulated in the gestagen-treated groups as compared to placebo that might be involved in thrombotic events

2014-06-30 | GSE57972 | GEO

Differential effects of progesterone and medroxyprogesterone acetate on normal breast cells

2011-05-30 | GSE23500 | GEO

Mus musculus

Project description:Analysis of aortic gene expression in ovariectomized ApoE-deficient mice substituted with medroxyprogesterone acetate, norethisterone acetate or placebo

| PRJNA248622 | ENA

Response of Escherichia coli to acetate concentration during growth on glucose

Project description:E. coli MG1655 was grown on M9 minimal medium supplemented with 15mM glucose and either 0, 10, 50 or 100 mM acetate. Transcriptomic analysis from exponential growing cells was performed using Agilent microarray A-MEXP-2232. This work was done with the help of students during practicals. Consequently, it is of special interest since we have four biological replicates done over four years and two technical replicates per biological replicate (n=8 per condition). We therefore thank all the INSA-Toulouse students that participated in this project: Leidy Caraballo, Xavier Caron, Pauline Chanut, Sarah Guiziou, Ngoc Thu Hang Pham, Diane Barbay, Céline Ben Hassen, Thomas Cerutti, Cécile Roland, Sarah Srour, Audrey Baylet, Mathilde Beraud, Claudie Bosc, Lilas Courtot, Violaine Dolfo, Anna Kaci, Manon Chevallot-Beroux, Sarah Colom, Fanny Leclerc, Zihan Liao, Grégoire Quinet and Mélina Vaurs.

2020-08-21 | E-MTAB-9086 | biostudies-arrayexpress

Response of Escherichia coli to acetate in presence of 100 mM methyl α-D-glucopyranoside

Project description:This experiment was performed to challenge E. coli response to acetate when its growth rate flux is reduced by 40% with 100 mM methyl α-D-glucopyranoside. Control without inhibition in the exact same conditions was already published on ArrayExpress database (accession number E-MTAB-9086) and could be used for comparison purpose. Here, E. coli BW25113 strain was grown in M9 medium complemented with 15 mM glucose, 100 mM αMG, and 0, 10, 50 or 100 mM sodium acetate. The cells were grown to mid-exponential phase in shake flasks at 37 °C and 200 rpm, in 50 mL of medium before proceeding to RNA extraction and subsequent microarray analysis. Three to four replicates (with two to three biological replicates) were analyzed for each condition. We thank all the INSA-Toulouse students that participated in this project: Lola Blayac, Romane Ducloux, Benjamin Jung, Oliver Larousse, Leyre Sarrias, Arno Bruel, Alexis Charbinat, Justine Dumas-Perdriau, Solène Frapard, Sophie Germain, Nicolas Papadopoulos, Marine Rodeghiero & Auriane Thomas.

2022-09-23 | E-MTAB-11717 | biostudies-arrayexpress

Combinatorial effects of estrogen with progestogens or androgen in the breast cancer cell line ZR-75-1

Project description:Cells were cotreated with dihydrotestosterone, progesterone or medroxyprogesterone acetate and estrdiol to assess the combinatorial effects of hormone exposure in breast cancer cells Cells were plated in hormone stripped media for 56h, followed by treatment for 16h with 10nM of the nominated hormone(s)

2015-10-18 | E-GEOD-61368 | biostudies-arrayexpress

Differential effects of progesterone and medroxyprogesterone acetate on normal breast cells

Project description:Comparison between the effects of progesterone (P4) and medroxyprogesterone acetate (MPA) combined to estradiol (E2) on gene expression in normal breast cells to provide insight on their possible different impact on breast cancer risk in vivo. Total RNA obtained from cultures of normal human breast epithelial cells (HBE) under E2, E2+MPA and E2+P4 six hour treatments, compared to untreated HBE cells

2011-05-30 | E-GEOD-23500 | biostudies-arrayexpress

Endometrial cancer cell line with acquired resistance to medroxyprogesterone acetate

Project description:Endometrial cancer cell line with acquired resistance to medroxyprogesterone acetate

| PRJNA497188 | ENA

Acetate oxidation

Project description:Acetate oxidation

| PRJNA1033353 | ENA

Evaluation of NHS-Acetate and DEPC labelling for determination of solvent accessible amino acid residues in protein complexes

Project description:The structural analysis of proteins and protein complexes is key for understanding their function and regulation. In order to gain structural information, we examined protein surface labelling using NHS-Acetate and DEPC following an MS-based workflow. Using two model proteins we characterise the obtained mass spectra and explore the applicability of a quantitative approach for determining of solvent accessible amino acid residues on the surface of proteins and protein complexes. The modified peptides were analysed by LC-MS/MS and processed using MaxQuant for identification and intensity determination of labelled and unlabelled peptides.

2020-05-05 | PXD015940 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data