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Required Enhancer: Matrin-3 Structure Interactions for Homeodomain Transcription Programs

ABSTRACT: Study of the POU-homeodomain transcription factor, has revealed that, binding of Pit1-occupied enhancers to a nuclear matrin-3-rich network/architecture is a key event in effective activation of the Pit1-regulated enhancer / coding gene transcriptional program. All ChIP-seq(s) were designed to understand the unique features, associated molecular mechanisms and functions of Matrin3 in the Homeodomain transcription study. Gro-seq Samples are used to detect gene expression between knockdown control and knockdown two key factors in our study.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Qi Ma

PROVIDER: E-GEOD-58009 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Required enhancer-matrin-3 network interactions for a homeodomain transcription program.

Skowronska-Krawczyk Dorota D Ma Qi Q Schwartz Michal M Scully Kathleen K Li Wenbo W Liu Zhijie Z Taylor Havilah H Tollkuhn Jessica J Ohgi Kenneth A KA Notani Dimple D Kohwi Yoshinori Y Kohwi-Shigematsu Terumi T Rosenfeld Michael G MG

Nature 20140803 7521

Homeodomain proteins, described 30 years ago, exert essential roles in development as regulators of target gene expression; however, the molecular mechanisms underlying transcriptional activity of homeodomain factors remain poorly understood. Here investigation of a developmentally required POU-homeodomain transcription factor, Pit1 (also known as Pou1f1), has revealed that, unexpectedly, binding of Pit1-occupied enhancers to a nuclear matrin-3-rich network/architecture is a key event in effecti ...[more]

PMID: 25119036

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