Unknown,Transcriptomics,Genomics,Proteomics

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Separation of mouse ES cell-derived mesodermal cells based on the expression of Flk1, PDGFRa and CAR.

ABSTRACT: We found that mouse ES cell-derived Flk1+ cells could be subdivided into three population by the expression of PDGFRa and CAR (Flk1+PDGFRa-CAR-, Flk1+PDGFRa-CAR+, and Flk1+PDGFRa+CAR+). Therefore, global gene expression analysis was perfomed by microarray to characterize these mesodermal subsets. RNA isolated from five separate experiments was pooled and used for comparison

ORGANISM(S): Mus musculus

SUBMITTER: Satoshi Kondo 

PROVIDER: E-GEOD-58170 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Expression of coxsackievirus and adenovirus receptor separates hematopoietic and cardiac progenitor cells in fetal liver kinase 1-expressing mesoderm.

Tashiro Katsuhisa K   Hirata Nobue N   Okada Atsumasa A   Yamaguchi Tomoko T   Takayama Kazuo K   Mizuguchi Hiroyuki H   Kawabata Kenji K  

Stem cells translational medicine 20150311 5

In developing embryos or in vitro differentiation cultures using pluripotent stem cells (PSCs), such as embryonic stem cells and induced pluripotent stem cells, fetal liver kinase 1 (Flk1)-expressing mesodermal cells are thought to be a heterogeneous population that includes hematopoietic progenitors, endothelial progenitors, and cardiac progenitors. However, information on cell surface markers for separating these progenitors in Flk1⁺ cells is currently limited. In the present study, we show th  ...[more]

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