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Multi-Omic Integrated networks connect DNA methylation and miRNA with skeletal muscle plasticity to chronic exercise in type 2 diabetic obesity [mRNA data]

ABSTRACT: Analysis of skeletal muscle gene expression from type 2 diabetic volunteers before and after 16 weeks of chronic exercise training (two groups, one undergoing aerobic ecercise and the other resistance training exercise) A biopsy was collected from the right Vastus Lateralis under local anaesthesia and RNA was isolated from muscle (~10 mg) using mirVana™ Kit , 200 ng RNA from each sample was used for mRNA expression profiling using HumanHT-12_v4_BeadChip (Illumina). Arrays were scanned using HiScanSQ System. Decoded images were analyzed by GenomeStudio™ (Illumina).

ORGANISM(S): Homo sapiens

SUBMITTER: David Rowlands

PROVIDER: E-GEOD-58249 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Multi-Omic Integrated networks connect DNA methylation and miRNA with skeletal muscle plasticity to chronic exercise in type 2 diabetic obesity [Methylation data]

Project description:Analysis of skeletal muscle DNA methylation from type 2 diabetic volunteers before and after 16 weeks of chronic exercise training (two groups, one undergoing aerobic excercise and the other resistance training exercise) A biopsy was collected from the right Vastus Lateralis under local anaesthesia andGenomic DNA was extracted from 5-10 mg muscle, Bisulphite conversion (Illumina) was checked using methylation specific PCR. 4 ?l of bisulphite-converted DNA was used for hybridization on Infinium Human Methylation 450 BeadChip (Illumina)

2014-09-10 | E-GEOD-58280 | biostudies-arrayexpress

Multi-Omic Integrated networks connect DNA methylation and miRNA with skeletal muscle plasticity to chronic exercise in type 2 diabetic obesity [miRNA data]

Project description:Analysis of skeletal muscle miRNA expression from type 2 diabetic volunteers before and after 16 weeks of chronic exercise training (two groups, one undergoing aerobic ecercise and the other resistance training exercise) A biopsy was collected from the right Vastus Lateralis under local anaesthesia and RNA was isolated from muscle (~10 mg) using mirVanaM-bM-^DM-" Kit , Low molecular weight RNA was biotin-labeled with FlashTagM-bM-^DM-" Biotin HSR RNA Labeling Kit. 21.5 M-BM-5l of high-quality biotin-labeled miRNA sample was hybridized to Affymetrix Gene-ChipM-BM-. miRNA 2.0, stained on Affymetrix Fluidics station 400 and scanned with a Hewlett Packard G2500A gene Array Scanner. AffymetrixM-BM-. miRNA QC Tool v 1.1.1.0 was used for the data summarization, normalization, and microarray quality control.

2014-09-10 | E-GEOD-58248 | biostudies-arrayexpress

Multi-Omic Integrated networks connect DNA methylation and miRNA with skeletal muscle plasticity to chronic exercise in type 2 diabetic obesity [Methylation data]

2014-09-10 | GSE58280 | GEO

Multi-Omic Integrated networks connect DNA methylation and miRNA with skeletal muscle plasticity to chronic exercise in type 2 diabetic obesity [mRNA data]

Project description:Analysis of skeletal muscle gene expression from type 2 diabetic volunteers before and after 16 weeks of chronic exercise training (two groups, one undergoing aerobic ecercise and the other resistance training exercise)

2014-09-10 | GSE58249 | GEO

Multi-Omic Integrated networks connect DNA methylation and miRNA with skeletal muscle plasticity to chronic exercise in type 2 diabetic obesity [miRNA data]

2014-09-10 | GSE58248 | GEO

Age-dependent impact of two exercise training regimens on genomic and metabolic remodeling in skeletal muscle and liver of male mice

Project description:How skeletal muscle adapts to different types of exercise intensity with age is not known. Adult and old C57BL/6 male mice were assigned to one of three groups: sedentary, daily high-intensity intermittent training (HIIT), or moderate intensity continuous training (MICT) for 4 weeks, compatible with the older group’s exercise capacity. Improvements in body composition, fasting blood glucose, and muscle strength were mostly observed in the MICT old group, while effects of HIIT training in adult and old animals was less clear. Skeletal muscle exhibited structural and functional adaptations to exercise training, as revealed by electron microscopy, OXPHOS assays, respirometry, and muscle protein biomarkers. Transcriptomics analysis of gastrocnemius muscle combined with liver and serum metabolomics unveiled an age-dependent metabolic remodeling in response to exercise training. These results support a tailored exercise prescription approach aimed at improving health and ameliorating age-associated loss of muscle strength and function in the elderly.

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Project description:Background: Exercise has a positive effect on overall health. This study was performed to get an overview of the effects of mixed exercise training on skeletal muscl 18 middle-aged men performed 12 weeks of exercise training (2x endurance training and 1x resistance training), muscle biopsies were taken at baseline and 3 days after the last training session

2013-12-24 | E-GEOD-53598 | biostudies-arrayexpress

Gene expression changes in skeletal muscle following aerobic exercise training

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Dynamics of the human skeletal muscle transcriptome in response to exercise training - part 2

Project description:In the present study 23 participants completed three months of supervised aerobic exercise training of one leg (training period 1) followed by 9 months of rest before 12 of the participants completed a second exercise training period (training period 2) of three months of both legs. Skeletal muscle biopsies have been collected before and after the training periods. We have compared trained leg with untrained leg and studied gene and isoform expression. Additional samples included in this study has been previously submitted (GEO accession number GSE58387 and GSE60590). Analyze of transcriptome in skeletal muscle biopsy samples in response to exercise training in 23 participants in total (in addition to data previously submitted GEO accession number GSE58387 and GSE60590). Biopsy is collected from skeletal muscle before and after training period.

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