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The role of AR-associated factors in androgen signaling

ABSTRACT: Prostate cancer is the most common cancer in men and AR downstream signalings promote prostate cancer cell proliferation. We identified androgen-regulated genes, CTBP2, FOXP1 and RUNX1. These factors interact with AR ligand dependently. In order to investigate androgen-regulated gene functions in prostate cancer cells, we performed gene expression in AR-positive prostate cancer cell lines after siRNA treatment. We also treated cells with vehicle or androgen to analyzed the effects of these genes on AR function. Observation of androgen dependent gene expression changes after treatment with siRNAs targeting FOXP1, CTBP2 and FOXA1 with microarray.

ORGANISM(S): Homo sapiens

SUBMITTER: Ken-ichi Takayama

PROVIDER: E-GEOD-58309 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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CtBP2 modulates the androgen receptor to promote prostate cancer progression.

Takayama Ken-Ichi K Suzuki Takashi T Fujimura Tetsuya T Urano Tomohiko T Takahashi Satoru S Homma Yukio Y Inoue Satoshi S

Cancer research 20140916 22

The androgen receptor (AR) is the key driver of both early and advanced prostate cancer, making a complete understanding of its regulation important. Here, we report the identification of multiple AR-binding sites in the gene encoding the transcription factor CtBP2 (carboxyl terminal-binding protein), genetic variations of which have been associated with prostate cancer susceptibility. Notably, we found that SNPs in the human CTBP2 gene that were associated with prostate cancer development were ...[more]

PMID: 25228652

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