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Expression data from mouse colon dendritic cell subsets

ABSTRACT: Characterization of colon CD11chigh/MHCII+ myeloid cell subsets Colon lamina propria MHC+CD11chigh cells were sorted on the basis of CD103 and CD11b expression as follows: CD103+CD11b-, CD103+CD11b+, CD103-CD11b+.

ORGANISM(S): Mus musculus

SUBMITTER: jinmiao chen

PROVIDER: E-GEOD-58446 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Intestinal CD103(+)CD11b(-) dendritic cells restrain colitis via IFN-γ-induced anti-inflammatory response in epithelial cells.

Muzaki A R B M AR Tetlak P P Sheng J J Loh S C SC Setiagani Y A YA Poidinger M M Zolezzi F F Karjalainen K K Ruedl C C

Mucosal immunology 20150715 2

A crosstalk between commensals, gut immune cells, and colonic epithelia is required for a proper function of intestinal mucosal barrier. Here we investigated the importance of two distinct intestinal dendritic cell (DC) subsets in controlling intestinal inflammation. We show that Clec9A-diphtheria toxin receptor (DTR) mice after depletion of CD103(+)CD11b(-) DCs developed severe, low-dose dextran sodium sulfate (DSS)-induced colitis, whereas the lack of CD103(+)CD11b(+) DCs in Clec4a4-DTR mice d ...[more]

PMID: 26174764

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Inflammation switches the differentiation program of Ly6Chi monocytes from anti-inflammatory macrophages to inflammatory dendritic cells in the colon

Project description:Dendritic cells (DCs) and macrophages (MPs) are important for immunological homeostasis in the colon. We found that F4/80hi CX3CR1hi (CD11b+CD103-) cells account for 80% of mouse colonic lamina propria (cLP) MHC-IIhi cells. Both CD11c+ and CD11c- cells within this population were identified as MPs based on multiple criteria, including a MP transcriptome revealed by microarray analysis. These MPs constitutively released high levels of IL-10 at least partially in response to the microbiota via an MyD88-independent mechanism. In contrast, cells expressing low to intermediate levels of F4/80 and CX3CR1 were identified as DCs, based on phenotypic and functional analysis and comprise three separate CD11chi cell populations: CD103+CX3CR1-CD11b- DCs, CD103+CX3CR1-CD11b+ DCs and CD103-CX3CR1intCD11b+ DCs. In non-inflammatory conditions, Ly6Chi monocytes differentiated primarily into CD11c+, but not CD11c- MPs. In contrast, during colitis, Ly6Chi monocytes massively invaded the colon and differentiated into pro-inflammatory CD103-CX3CR1intCD11b+ DCs, which produced high levels of IL-12, IL-23, iNOS and TNF. These findings demonstrate the dual capacity of Ly6Chi blood monocytes to differentiate into either regulatory MPs or inflammatory DCs in the colon, and that the balance of these immunologically antagonistic cell types is dictated by microenvironmental conditions. FACS sorted expression from normal controls

2011-12-21 | E-GEOD-27859 | biostudies-arrayexpress

Expression of Spi-C in intestinal CX3CR1high macrophages

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Inflammation switches the differentiation program of Ly6Chi monocytes from anti-inflammatory macrophages to inflammatory dendritic cells in the colon

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2016-03-27 | E-GEOD-79631 | biostudies-arrayexpress

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2015-01-06 | E-GEOD-56438 | biostudies-arrayexpress

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2015-08-01 | E-GEOD-68399 | biostudies-arrayexpress

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2010-03-24 | E-GEOD-17322 | biostudies-arrayexpress

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