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Genome-wide Oct4 binding profile in lung cancer A549 cells

ABSTRACT: Oct4 stemness gene encoding a transcription factor has been shown to overexpress in cancers. However, precise mechanisms of Oct4 relevant to transcriptional reprogramming leading to somatic cancer progression remain unclear. To address the Oct4-mediated transcriptional program in lung cancer, we integrated genome-wide Oct4 binding profiles from chromatin-immunoprecipitation sequencing and ENCODE datasets. We identified that Oct4 occupied at functional promoter and enhancer regions of genes which play key roles in several signaling pathways involving tumorigenesis. Genome-wide Oct4 binding sites were identified via chromatin immunoprecipitation-sequencing analysis of vecoter control and stably Oct4-overexpressing A549 lung cancer cells. ChIP-seq analyses were performed in duplicated samples using Applied Biosystems SOLiD system.

ORGANISM(S): Homo sapiens

SUBMITTER: Yi-Ching Wang

PROVIDER: E-GEOD-58462 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Global Oct4 target gene analysis reveals novel downstream PTEN and TNC genes required for drug-resistance and metastasis in lung cancer.

Tang Yen-An YA Chen Chi-Hsin CH Sun H Sunny HS Cheng Chun-Pei CP Tseng Vincent S VS Hsu Han-Shui HS Su Wu-Chou WC Lai Wu-Wei WW Wang Yi-Ching YC

Nucleic acids research 20150121 3

Overexpression of Oct4, a stemness gene encoding a transcription factor, has been reported in several cancers. However, the mechanism by which Oct4 directs transcriptional program that leads to somatic cancer progression remains unclear. In this study, we provide mechanistic insight into Oct4-driven transcriptional network promoting drug-resistance and metastasis in lung cancer cell, animal and clinical studies. Through an integrative approach combining our Oct4 chromatin-immunoprecipitation seq ...[more]

PMID: 25609695

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