Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Gene expression profiles for Pancreatic ductal adenocarcinoma cell lines and their corresponding normal and fresh frozen tissues.

ABSTRACT: To demonstrate that the generated cell lines represent the parental tumours we compared the mRNA expression patterns obtained from the generated cell lines, the original tumours, as well as non-tumorigenic pancreatic tissue from the same patients Total RNA was isolated from the cell lines PpaX1, PpaX6 and PpaX8 as well as their corresponding original tumours and adjacent normal tissue from patient Ppa6 and Ppa8 and analysed by microarray analysis using the 60K agilent platform. The expression profiles were background corrected and quantile normalized. Filtering of controls, unannotated genes and low expressed probes was done. 10,947 genes were used to demonstarte the relationship between the cell lines and parental tumors.

ORGANISM(S): Homo sapiens

SUBMITTER: Vandana Sandhu

PROVIDER: E-GEOD-58561 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Publications

Generation and characterisation of novel pancreatic adenocarcinoma xenograft models and corresponding primary cell lines.

Wennerström Anna B AB Lothe Inger Marie Bowitz IM Sandhu Vandana V Kure Elin H EH Myklebost Ola O Munthe Else E

PloS one 20140822 8

Pancreatic adenocarcinoma is one of the most lethal cancer types, currently lacking efficient treatment. The heterogeneous nature of these tumours are poorly represented by the classical pancreatic cell lines, which have been through strong clonal selection in vitro, and are often derived from metastases. Here, we describe the establishment of novel pancreatic adenocarcinoma models, xenografts and corresponding in vitro cell lines, from primary pancreatic tumours. The morphology, differentiation ...[more]

PMID: 25148029

Similar Datasets

Project description:Despite the high prevalence and poor outcome of patients with metastatic lung cancer, the mechanisms of tumour progression and metastasis remain largely uncharacterized. We modelled human lung adenocarcinoma, which frequently harbours activating point mutations in KRAS1 and inactivation of the p53-pathway2, using conditional alleles in mice3-5. Lentiviral-mediated somatic activation of oncogenic Kras and deletion of p53 in the lung epithelial cells of KrasLSL-G12D/+;p53flox/flox mice initiates lung adenocarcinoma development4. Although tumours are initiated synchronously by defined genetic alterations, only a subset become malignant, suggesting that disease progression requires additional alterations. Identification of the lentiviral integration sites allowed us to distinguish metastatic from non-metastatic tumours and determine the gene expression alterations that distinguish these tumour types. Cross-species analysis identified the NK-2 related homeobox transcription factor Nkx2-1 (Ttf-1/Titf1) as a candidate suppressor of malignant progression. In this mouse model, Nkx2-1-negativity is pathognomonic of high-grade poorly differentiated tumours. Gain- and loss-of-function experiments in cells derived from metastatic and non-metastatic tumours demonstrated that Nkx2-1 controls tumour differentiation and limits metastatic potential in vivo. Interrogation of Nkx2-1 regulated genes, analysis of tumours at defined developmental stages, and functional complementation experiments indicate that Nkx2-1 constrains tumours in part by repressing the embryonically-restricted chromatin regulator Hmga2. While focal amplification of NKX2-1 in a fraction of human lung adenocarcinomas has focused attention on its oncogenic function6-9, our data specifically link Nkx2-1 downregulation to loss of differentiation, enhanced tumour seeding ability, and increased metastatic proclivity. Thus, the oncogenic and suppressive functions of Nkx2-1 in the same tumour type substantiate its role as a dual function lineage factor. 23 cell lines derived from primary tumor or metastasis. 6 samples analyzed to determine the effect of Nkx2-1 knockdown on gene expression

Dataset Information

Gene expression profiles for Pancreatic ductal adenocarcinoma cell lines and their corresponding normal and fresh frozen tissues.

Publications

Generation and characterisation of novel pancreatic adenocarcinoma xenograft models and corresponding primary cell lines.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets