Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Gene expression profiles of mouse embryonic stem cells (mESCs) after genetic depletion of Id1 and knock-down of Zrf1

ABSTRACT: Id proteins are dominant negative regulators within the HLH family of proteins. In embryonic stem cells (ESCs), Id1 and Id3 maintain the pluripotent state by preventing neural differentiation. The Id1-interacting protein Zrf1 plays a crucial role as a chromatin-bound factor in specification of the neural fate from ESCs. Here, we show that Id1 blocks Zrf1 recruitment to chromatin, thus preventing the activation of neural genes during ESC differentiation. Moreover, genetic deletion of Id1 in ESCs caused misexpression of more than 6000 genes. Interestingly, the expression of almost half of those genes was restored upon further depletion of Zrf1. We therefore identified Zrf1 as a transcriptional regulator downstream of Id1 in ESCs. In Id1KO mESCs, Zrf1 expression was depleted by using shRNAs. Four replicates corresponding to four independent biological samples per group were collected.

ORGANISM(S): Mus musculus

SUBMITTER: Enrique Blanco

PROVIDER: E-GEOD-58627 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Direct interaction between Id1 and Zrf1 controls neural differentiation of embryonic stem cells.

Aloia Luigi L Gutierrez Arantxa A Caballero Juan Martin JM Di Croce Luciano L

EMBO reports 20141031 1

Id proteins are dominant-negative regulators within the HLH family of proteins. In embryonic stem cells (ESCs), Id1 and Id3 maintain the pluripotent state by preventing neural differentiation. The Id1-interacting protein Zrf1 plays a crucial role as a chromatin-bound factor in specification of the neural fate from ESCs. Here, we show that Id1 blocks Zrf1 recruitment to chromatin, thus preventing the activation of neural genes in ESCs. Upon differentiation, Id1 expression decreases thus inducing ...[more]

PMID: 25361733

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Dataset Information

Gene expression profiles of mouse embryonic stem cells (mESCs) after genetic depletion of Id1 and knock-down of Zrf1

Publications

Direct interaction between Id1 and Zrf1 controls neural differentiation of embryonic stem cells.

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