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Genome-wide analysis of the transcriptomes of mouse embryonic stem cells (mESC) maintained in different indicated conditions.

ABSTRACT: Analysis of genes involved in the mESC maintainance between different conditions of growth. The hypothesis tested in the present study was that Myc can sustain mESC self renewal and pluripotency. Results provide important information on the mechanism by which Myc supports mESC self renewal, in comparison with the routinely used LIF+serum culture condition, such as a Myc-dependent specific transcriptional program, which involves induction of an alternative Core Regulatory Network, modulation of signallign pathways (Wnt/bcat) and repression of developmental genes. Total RNA obtained from mESC growth 3 days in the indicated condition compared to the routinely used LIF+serum culture condition. Total RNA obtained from EpiSC growth 3 days in standard condition were also used as control for primed stem cell state.

ORGANISM(S): Mus musculus

SUBMITTER: Alessio Zippo

PROVIDER: E-GEOD-58656 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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