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Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells

ABSTRACT: To characterize the differentiation by Sox2 KO, we performed microarray analyses of mouse ES cell line 2TS22C during the time-course being induced of Sox2 KO Inducible Sox2 KO cell line 2TS22C were assigned to eight experimental groups and cultured in GMEM medium (SIGMA) with LIF at 37Â°C in an atmosphere of 5% CO2 until the specified time point before the harvest: (1)for controls, cultured in the absence of Tc; (2) for 3h after the administration of Tc; (3) for 6h; (4) for 12h; (5) for 24h; (6) for 48h; (7) for 72h; (8) for 96h.

ORGANISM(S): Mus musculus

SUBMITTER: Kazue Takahashi

PROVIDER: E-GEOD-5895 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells.

Masui Shinji S Nakatake Yuhki Y Toyooka Yayoi Y Shimosato Daisuke D Yagi Rika R Takahashi Kazue K Okochi Hitoshi H Okuda Akihiko A Matoba Ryo R Sharov Alexei A AA Ko Minoru S H MS Niwa Hitoshi H

Nature cell biology 20070521 6

The pluripotency of embryonic stem (ES) cells is thought to be maintained by a few key transcription factors, including Oct3/4 and Sox2. The function of Oct3/4 in ES cells has been extensively characterized, but that of Sox2 has yet to be determined. Sox2 can act synergistically with Oct3/4 in vitro to activate Oct-Sox enhancers, which regulate the expression of pluripotent stem cell-specific genes, including Nanog, Oct3/4 and Sox2 itself. These findings suggest that Sox2 is required by ES cells ...[more]

PMID: 17515932

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