Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Telomeric ORFs (TLOs) in pathogenic Candida spp. encode Mediator subunits that regulate the transcription of distinct subsets of genes

ABSTRACT: Analysis of the role of the Candida dubliniensis Telomeric (TLO) genes and MED3, encoding subunits of Mediator, in regulating transcription The transcriptional response of a Candida dubliniensis TLO1/TLO2 double (null) mutant was analysed. Reintegrant strains harboring TLO1 or TLO2 were compared to this null mutant to elucidate the individual role of each ORF. The role of MED3 was compared also. 9 experiemental parameters were analysed, each carried out in triplicate or quadruplicate

ORGANISM(S): Candida dubliniensis

SUBMITTER: Gary Moran

PROVIDER: E-GEOD-59113 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Telomeric ORFs (TLOs) in pathogenic Candida spp. encode Mediator subunits that regulate the transcription of distinct subsets of genes

Project description:Analysis of the role of the Candida dubliniensis Telomeric (TLO) genes and MED3, encoding subunits of Mediator, in regulating transcription The transcriptional response of a Candida dubliniensis TLO1/TLO2 double (null) mutant was analysed. Reintegrant strains harboring TLO1 or TLO2 were compared to this null mutant to elucidate the individual role of each ORF. The role of MED3 was compared also.

2014-07-11 | GSE59113 | GEO

Telomeric ORFs (TLOs) in Candida spp. encode Mediator subunits that regulate distinct virulence traits

Project description:Analysis of the interaction of Candida dubliniensis protein Tlo1p with DNA by ChIP-chip HA-tagged Tlo1p in Candida dubliniensis was used to identify Tlo1 interacting regions of DNA in global ChIP-chip analysis

2014-08-06 | GSE60173 | GEO

Telomeric ORFs (TLOs) in Candida spp. encode Mediator subunits that regulate distinct virulence traits

2014-08-06 | E-GEOD-60173 | biostudies-arrayexpress

Transcriptional response of Candida dubliniensis during hypha formation and environmental change

Project description:Transcriptional response of Candida dubliniensis during hypha formation and environmental change (temperature, pH, density and nutrients). Transcript profiling of C. dubliniensis identified a core shared transcriptional response with C. albicans during hypha formation and growth at alkaline pH. However, C. albicans expresses several unique hypha-specific genes, including ALS3, HYR1 and SAP4 and 5. Transcript profiling also revealed a novel role for NRG1 in regulating ferric reductases in C. dubliniensis. 8 experimental parameters were analysed, each carried out in quadruplicate. Dye-swaps were performed.

2010-02-26 | E-GEOD-20537 | biostudies-arrayexpress

Transcriptional profile analysis of Candida dubliniensis chlamydospores compared to yeast cells

Project description:In order to investigate the biology of chlamydospores, we compared global gene expression in C. dubliniensis yeast cells and chlamydospores. This revealed that 11% of the genes greater than 2-fold upregulated in chlamydospores were genes regulated by the transcriptional repressor Nrg1. These mainly included genes involved in glucose metabolism and glucose transport. Other upregulated genes included genes involved in stress response, cell cycle control, chromatin regulation, chromosome rearrangements, DNA repair-recombination and pheromone response. Genes related to mitochondrial respiratory functions and biogenesis were found to be downregulated in chlamydospores. Our data confirm that chlamydospores represent a specialised growth form of C. albicans and C. dubliniensis. 2 experimental parameters were analysed (chlamydospores, yeast cells). Three biological replicates each, one technical replicate.

2010-09-01 | E-GEOD-21173 | biostudies-arrayexpress

Expression profiling of C. albicans and C. dubliniensis in reconstituted human epithelium

Project description:This SuperSeries is composed of the following subset Series: GSE13318: Expression profiling of Candida albicans and Candida dubliniensis in reconstituted human oral epithelium 30 min p.i. GSE13345: Expression profiling of Candida albicans and Candida dubliniensis in reconstituted human oral epithelium 90 min p.i. GSE13352: Comparative expression profiling in Candida albicans and Candida dubliniensis in inocula, RHE, and PCF cultures Refer to individual Series

2010-05-16 | E-GEOD-13593 | biostudies-arrayexpress

Transcription profiling of Candida dubliniensis and Candida albicans under stress conditions

Project description:While Candida dubliniensis and Candida albicans are very close related species, the later is a far more successful yeast pathogen. Several explanations have been pointed out such as discrepancies in fitness, morphogenesis, adherence, or stress resistance. In this study, we investigate the transcriptional reshuffling of C. albicans and C. dubliniensis under conditions that highlight the difference of stress resistances between these strains.

2008-12-01 | E-MEXP-1650 | biostudies-arrayexpress

Comparative expression profiling in Candida albicans and Candida dubliniensis in inocula, RHE, and PCF cultures

Project description:The opportunistic human pathogens, Candida albicans and Candida dubliniensis, are closely related species displaying large differences in virulence, but the reasons for these differences are elusive. Microarray-based comparative analysis of global gene expression in the two species incubated on reconstituted human oral epithelium (RHE) was used to identify specific and common changes in gene expression and find novel C. albicans virulence genes Comparative analysis of global gene expression in Candida albicans SC5314 and Candida dubliniensis CD36 in reconstituted human oral epithelium (RHE), polycarbonate filter (PCF; used as RHE support matrix) 30 and 90 min postinoculation, and in cultures used as inocula (0 min). Gene expression in C. albicans and C. dubliniensis was assessed by co-hybridizations matched by treatments. Performed in two or three biological replicates with reciprocal dye swaps for each biological replicate. Gene expression of Candida cells on RHE was normalized to gene expression in reference control (PCF); log2 ratios were calculated by dividing spot intensity of experimental (C. albicans) by that of the reference control (C. dubliniensis).

2010-05-16 | E-GEOD-13352 | biostudies-arrayexpress

Lineage-specific selection and the evolution of virulence in Candida albicans

Project description:This data was generated to compare genome-wide expression differences between a major fungal pathogen of humans, Candida albicans and its less pathogenic relative, Candida dubliniensis, using interspecies hybrids to systematically identify cis-regulatory adaptations.

2021-03-31 | GSE168112 | GEO

Transcriptional response of Candida dubliniensis during hypha formation and environmental change

2010-02-27 | GSE20537 | GEO