Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Expression data from heterotopically grafted mouse small intestinal epithelium and the normal colonic epithelium

ABSTRACT: Epithelia of small and large intestines differ in their structures and functions. Such heterogeneity between these two epithelial tissues might be controlled by both epithelium-intrinsic and -extrinsic programs. By employing the cell transplantation technique developed in our laboratory, we investigated how adult SI epithelial cells behave when heterotopically transplanted onto colon. Then the gene expression profiles of small intestinal epithelium heterotopically transplanted onto colon and control colonic epithelium were compared. We used laser capture microdissection and microarray analysis to compare the global gene expression profiles of small intestinal epithelium in the heterotopically grafted tissues and control colonic epithelium. Dissected epithelia obtained from serial sections (~ 30 sections) of each specimen were combined and then total RNA extraction was performed. After calculating RNA Integrity Number (RIN), we subjected one graft sample and one control colon sample, which showed the highest RIN value, for the following gene expression analysis.

ORGANISM(S): Mus musculus

SUBMITTER: Tetsuya Nakamura

PROVIDER: E-GEOD-59410 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Small intestinal stem cell identity is maintained with functional Paneth cells in heterotopically grafted epithelium onto the colon.

Fukuda Masayoshi M Mizutani Tomohiro T Mochizuki Wakana W Matsumoto Taichi T Nozaki Kengo K Sakamaki Yuriko Y Ichinose Shizuko S Okada Yukinori Y Tanaka Toshihiro T Watanabe Mamoru M Nakamura Tetsuya T

Genes & development 20140801 16

To develop stem cell therapy for small intestinal (SI) diseases, it is essential to determine whether SI stem cells in culture retain their tissue regeneration capabilities. By using a heterotopic transplantation approach, we show that cultured murine SI epithelial organoids are able to reconstitute self-renewing epithelia in the colon. When stably integrated, the SI-derived grafts show many features unique only to the SI but distinct from the colonic epithelium. Our study provides evidence that ...[more]

PMID: 25128495

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Dataset Information

Expression data from heterotopically grafted mouse small intestinal epithelium and the normal colonic epithelium

Publications

Small intestinal stem cell identity is maintained with functional Paneth cells in heterotopically grafted epithelium onto the colon.

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