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Lactococcus lactis subsp. cremoris GE2-14 (GE2-14) vs Lactococcus lactis subsp. cremoris GE2-14 ?aguR (GE2-14 ?aguR)

ABSTRACT: Trancriptional profiling of GE2-14 ?aguR mutant strain in which the aguR gene of the agmatine deiminase cluster has been deleted. We compared the expression profiling of GE2-14 cells with the expression profiling of GE2-14 ?aguR mutant cells. The goal was to determine the effect of the aguR deletion on the transcriptional profiling of GE2-14 Two strains GE2-14 (reference) vs. GE2-14 ?aguR (test) cells in same conditions. Three biological replicates, two technical replicates and one dye swap.

ORGANISM(S): Lactococcus lactis subsp. cremoris GE214

SUBMITTER: Victor Ladero

PROVIDER: E-GEOD-59514 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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AguR, a Transmembrane Transcription Activator of the Putrescine Biosynthesis Operon in Lactococcus lactis, Acts in Response to the Agmatine Concentration.

Linares Daniel M DM Del Rio Beatriz B Redruello Begoña B Ladero Victor V Martin M Cruz MC de Jong Anne A Kuipers Oscar P OP Fernandez Maria M Alvarez Miguel A MA

Applied and environmental microbiology 20150626 18

Dairy industry fermentative processes mostly use Lactococcus lactis as a starter. However, some dairy L. lactis strains produce putrescine, a biogenic amine that raises food safety and spoilage concerns, via the agmatine deiminase (AGDI) pathway. The enzymatic activities responsible for putrescine biosynthesis in this bacterium are encoded by the AGDI gene cluster. The role of the catabolic genes aguB, aguD, aguA, and aguC has been studied, but knowledge regarding the role of aguR (the first gen ...[more]

PMID: 26116671

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Transcriptome profiling of Lactococcus lactis subsp. cremoris CECT 8666 in response to agmatine

Project description:The goal was to determine the effect of agmatine on the trancriptional profile of L. lactis CECT 8666 strain. For that we compared the expression profile of L. lactis CECT 8666 cells grown in culture medium supplemented with 20 mM agmatine with the expression profile of L. lactis CECT 8666 cells grown in culture medium without agmatine. L. lactis CECT 8666 cells grown in GalM17 medium (reference) compared to L. lactis CECT 8666 cells grown in GalM17 medium supplemented with 20 mM agmatine (test).

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Lactococcus lactis subsp. cremoris GE2-14 (GE2-14) vs Lactococcus lactis subsp. cremoris GE2-14 ΔaguR (GE2-14 ΔaguR)

Project description:Trancriptional profiling of GE2-14 ΔaguR mutant strain in which the aguR gene of the agmatine deiminase cluster has been deleted. We compared the expression profiling of GE2-14 cells with the expression profiling of GE2-14 ΔaguR mutant cells. The goal was to determine the effect of the aguR deletion on the transcriptional profiling of GE2-14

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