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Transcription profiling of mouse Th1 T cell clone and anergic Th1 T cell clone made anergic by plate-bound anti-CD3 antibody

ABSTRACT: compare gene expression profiles between normal and anergic T cells and identify upregulated genes in anergic T cells Experiment Overall Design: RNA from normal Th1 T cell clone and anergic Th1 T cell clone made anergic by plate-bound anti-CD3 antibody were isolated and amplified for microarray analysis

ORGANISM(S): Mus musculus

SUBMITTER: yuanyuan zha

PROVIDER: E-GEOD-5960 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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T cell anergy is reversed by active Ras and is regulated by diacylglycerol kinase-alpha.

Zha Yuanyuan Y Marks Reinhard R Ho Allen W AW Peterson Amy C AC Janardhan Sujit S Brown Ian I Praveen Kesavannair K Stang Stacey S Stone James C JC Gajewski Thomas F TF

Nature immunology 20061008 11

T cell anergy has been correlated with defective signaling by the GTPase Ras, but causal and mechanistic data linking defective Ras activity with T cell anergy are lacking. Here we used adenoviral transduction to genetically manipulate nonproliferating T cells and show that active Ras restored interleukin 2 production and mitogen-activated protein kinase signaling in T cells that were made anergic in vitro or in vivo. Diacylglycerol kinases (DGKs), which negatively regulate Ras activity, were up ...[more]

PMID: 17028589

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