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Chromatin state dynamics during blood formation (ChIP-Seq)

ABSTRACT: We develop a new ChIpseq method (iChIP) to profile chromatin states of low cell number samples. We use IChIP to profile the chromatin dynamics during hematopoiesis across 16 different cell types which include the principal hematopoietic progenitors Examination of 2 different histone modifications in 2 cell types.

ORGANISM(S): Mus musculus

SUBMITTER: Ido Amit

PROVIDER: E-GEOD-59636 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Immunogenetics. Chromatin state dynamics during blood formation.

Lara-Astiaso David D Weiner Assaf A Lorenzo-Vivas Erika E Zaretsky Irina I Jaitin Diego Adhemar DA David Eyal E Keren-Shaul Hadas H Mildner Alexander A Winter Deborah D Jung Steffen S Friedman Nir N Amit Ido I

Science (New York, N.Y.) 20140807 6199

Chromatin modifications are crucial for development, yet little is known about their dynamics during differentiation. Hematopoiesis provides a well-defined model to study chromatin state dynamics; however, technical limitations impede profiling of homogeneous differentiation intermediates. We developed a high-sensitivity indexing-first chromatin immunoprecipitation approach to profile the dynamics of four chromatin modifications across 16 stages of hematopoietic differentiation. We identify 48,4 ...[more]

PMID: 25103404

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| phs001704 | dbGaP

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