Unknown,Transcriptomics,Genomics,Proteomics

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MiR-222 is necessary for exercise-induced cardiac growth and protects against pathological cardiac remodeling.

ABSTRACT: miR-222 overexpression leads to promotion of proliferation and hypertrophy and inhibition of apoptosis in in primary neonatal rat ventricular cardiomyocytes (NRVMs). Isolated primary neonatal rat ventricular cardiomyocytes were plated in 6 cm BD Primaria tissue culture dishes. Transfection of microRNA precursors or scramble control (0.4 μM) was carried out using Lipofectamine RNAiMAX (Invitrogen) as recommended by the manufacturer. Forty-eight hours after transfection, RNAs from cultured cells and tissues were isolated with Tryzol (Invitrogen) following the manufacturesâ?? manuals. Total RNA was harvested and submitted to the Dana-Farber Cancer Institute Molecular Diagnostics Laboratory for assay. These results revealed miR-222 regulated gene expression in primary neonatal rat ventricular cardiomyocytes. Gene expression in NRVM cells tranfected with control scramble precursor (4 samples) or miR-222 precursor (4 smaples ) was evaluated using Affymetrix Rat Genome 230 v2.0.

ORGANISM(S): Rattus norvegicus

SUBMITTER: XIAOJUN LIU 

PROVIDER: E-GEOD-59641 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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miR-222 is necessary for exercise-induced cardiac growth and protects against pathological cardiac remodeling.

Liu Xiaojun X   Xiao Junjie J   Zhu Han H   Wei Xin X   Platt Colin C   Damilano Federico F   Xiao Chunyang C   Bezzerides Vassilios V   Boström Pontus P   Che Lin L   Zhang Chunxiang C   Spiegelman Bruce M BM   Rosenzweig Anthony A  

Cell metabolism 20150401 4

Exercise induces physiological cardiac growth and protects the heart against pathological remodeling. Recent work suggests exercise also enhances the heart's capacity for repair, which could be important for regenerative therapies. While microRNAs are important in certain cardiac pathologies, less is known about their functional roles in exercise-induced cardiac phenotypes. We profiled cardiac microRNA expression in two distinct models of exercise and found microRNA-222 (miR-222) was upregulated  ...[more]

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