Global gene expression analysis of FHB disease spreading mechanism in wheat

ABSTRACT: The Fusarium graminearum is responsible for Fusarium head blight (FHB) disease in wheat which makes unsuitable for end-use quality of wheat. For that reason several studies have been aiming to develop FHB resistant cultivars. In order to clarify possible wheat response mechanism, global gene expression analysis in response to the pathogen was undertaken. The study was conducted using three genotype of wheat, Japanese cultivar Nobeokabouzu-komugi (Highly resistant), cv. Sumai 3 (Resistant) and cv. Gamenya (Susceptible) which florets were inoculated Fusarium graminearum M-bM-^@M-^\H-3M-bM-^@M-^] strain during the flowering stage. For microarray analysis used samples of 3 and 7 days after inoculation (DAI) and Agilent wheat custom array 4x38k. Over 1400 genes were differentially up-regulated in Nobeokabouzu-komugi at 3 DAI followed by Sumai 3 and minimum expression in Gamenya, whereas at 7 DAI in Sumai 3 observed high expression and minimum in Gamenya. Further narrowing down by removing commonly expressed genes in three genotypes and grouping according to the gene function, identified differentially high expression of genes involved in detoxification process such as multidrug resistant rpoteins, UDP-glycosyltransferase and ABC transporters in Nobeokabouzu-komugi at 3 DAI. However in Sumai 3 found many localized defense-related genes such as peroxidase, proteases and genes involved in plant cell wall defense at 7 DAI. Following in silico analysis, revealed those genes were located in different chromosomes. This finding showed the differentiation of molecular response mechanism among the FHB resistant cultivars in response to the pathogen. F. graminearum inoculated (3 and 7 days after inoculation) and water inoculated Mock samples were used for cRNA synthesis and further hybridization with 3 biological replications. Total 12 samples per genotype

ORGANISM(S): Triticum aestivum

SUBMITTER: Alagu Manickavelu 

PROVIDER: E-GEOD-59721 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress